Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Vasopressin antibody [EPR20602] - BSA and Azide free (ab227050)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20602] to Vasopressin - BSA and Azide free
  • Suitable for: IHC-P, IHC-Fr, IP, Flow Cyt, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Vasopressin antibody [EPR20602] - BSA and Azide free
    See all Vasopressin primary antibodies

  • Description

    Rabbit monoclonal [EPR20602] to Vasopressin - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    In WB, FC and IP we detected Vasopressin only in overexpressing cells.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-Fr
    Mouse
    IHC-P
    Rat
    IP
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Mouse hypothalamus tissue.

  • General notes

    Ab227050 is the carrier-free version of ab213708. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab227050 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vasopressin antibody [EPR20602] - BSA and Azide free (ab227050)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vasopressin antibody [EPR20602] - BSA and Azide free (ab227050)

    Immunohistochemical analysis of paraffin-embedded rat hypothalamus tissue labeling Vasopressin with ab213708 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on neurons of rat paraventricular nucleus (PMID: 22822466, PMID: 17437647, PMID: 18773953). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213708).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Frozen sections) - Anti-Vasopressin antibody [EPR20602] - BSA and Azide free (ab227050)
    Immunohistochemistry (Frozen sections) - Anti-Vasopressin antibody [EPR20602] - BSA and Azide free (ab227050)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse brain tissue labeling Vasopressin with ab213708  at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive cytoplasmic staining in the neurons of paraventricular nucleus on mouse brain tissue section is observed.

    The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213708).

  • Immunohistochemistry (Frozen sections) - Anti-Vasopressin antibody [EPR20602] - BSA and Azide free (ab227050)
    Immunohistochemistry (Frozen sections) - Anti-Vasopressin antibody [EPR20602] - BSA and Azide free (ab227050)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen rat brain tissue labeling Vasopressin with ab213708  at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive cytoplasmic staining in the neurons of paraventricular nucleus on rat brain tissue section is observed.

    The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213708).

  • Flow Cytometry - Anti-Vasopressin antibody [EPR20602] - BSA and Azide free (ab227050)
    Flow Cytometry - Anti-Vasopressin antibody [EPR20602] - BSA and Azide free (ab227050)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with Vasopressin expression vector containing a myc-His-tag® cell line labeling Vasopressin with ab213708 at 1/500 dilution (right panel) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (left panel). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

    Co-stained with Alexa Fluor® 647 conjugated Myc-tag (Y-axis). Only Myc (+) population showed Vasopressin positive staining.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213708).

  • Immunoprecipitation - Anti-Vasopressin antibody [EPR20602] - BSA and Azide free (ab227050)
    Immunoprecipitation - Anti-Vasopressin antibody [EPR20602] - BSA and Azide free (ab227050)

    Vasopressin was immunoprecipitated from 0.35 mg of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with Vasopressin expression vector containing a myc-His-tag®, whole cell lysate with ab213708 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab213708 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: HEK-293T transfected with Vasopressin expression vector containing a myc-His-tag®, whole cell lysate 10 µg (Input). 

    Lane 2: ab213708 IP in HEK-293T transfected with Vasopressin expression vector containing a myc-His-tag®, whole cell lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab213708 in HEK-293T transfected with Vasopressin expression vector containing a myc-His-tag®, whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213708).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vasopressin antibody [EPR20602] - BSA and Azide free (ab227050)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vasopressin antibody [EPR20602] - BSA and Azide free (ab227050)

    Immunohistochemical analysis of paraffin-embedded mouse hypothalamus tissue labeling Vasopressin with ab213708 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on neurons of mouse paraventricular nucleus (PMID: 22822466, PMID: 17437647, PMID: 18773953). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213708).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-Vasopressin antibody [EPR20602] - BSA and Azide free (ab227050)
    Anti-Vasopressin antibody [EPR20602] - BSA and Azide free (ab227050)

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