Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Thyroglobulin antibody [EPR9730] - Low endotoxin, Azide free (ab229449)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9730] to Thyroglobulin - Low endotoxin, Azide free
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-Thyroglobulin antibody [EPR9730] - Low endotoxin, Azide free
    See all Thyroglobulin primary antibodies

  • Description

    Rabbit monoclonal [EPR9730] to Thyroglobulin - Low endotoxin, Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Mouse thyroid, rat thyroid, and Human thyroid lysates, Human thyroid gland follicular carcinoma tissue and Human thyroid gland papillary carcinoma tissue, TT cells

  • General notes

    ab229449 is the carrier-free version of ab156008.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroglobulin antibody [EPR9730] - Low endotoxin, Azide free (ab229449)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroglobulin antibody [EPR9730] - Low endotoxin, Azide free (ab229449)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human thyroid carcinoma tissue sections labeling Thyroglobulin with Purified ab156008 at 1:500 dilution (1.36 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab156008).

  • Flow Cytometry - Anti-Thyroglobulin antibody [EPR9730] - Low endotoxin, Azide free (ab229449)
    Flow Cytometry - Anti-Thyroglobulin antibody [EPR9730] - Low endotoxin, Azide free (ab229449)

    Flow Cytometry analysis of TT (Human thyroid carcinoma epithelial cell) cells labeling Thyroglobulin with purified ab156008 at 1:70 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab156008).

  • Immunocytochemistry/ Immunofluorescence - Anti-Thyroglobulin antibody [EPR9730] - Low endotoxin, Azide free (ab229449)
    Immunocytochemistry/ Immunofluorescence - Anti-Thyroglobulin antibody [EPR9730] - Low endotoxin, Azide free (ab229449)

    Immunocytochemistry/ Immunofluorescence analysis of TT (Human thyroid carcinoma epithelial cell) cells labeling Thyroglobulin with Purified ab156008 at 1:100 dilution. Cells were fixed in 100% Methanol. ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab156008).

  • Flow Cytometry - Anti-Thyroglobulin antibody [EPR9730] - Low endotoxin, Azide free (ab229449)
    Flow Cytometry - Anti-Thyroglobulin antibody [EPR9730] - Low endotoxin, Azide free (ab229449) This image is courtesy of an Abreview submitted by Sanjay Gawade.

    Unpurified ab156008 staining Thyroglobulin in mouse thyroid cells by Flow Cytometry. Cells were fixed with formaldehyde and permeabilized with permeabilization buffer. The sample was incubated with the primary antibody (1/100 in FACS buffer) for 30 minutes at 24°C. An Alexa Fluor® 647-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Gating Strategy: Epithlial cells. Red line shows unlabeled sample, blue line shows labeled sample.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab156008).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroglobulin antibody [EPR9730] - Low endotoxin, Azide free (ab229449)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroglobulin antibody [EPR9730] - Low endotoxin, Azide free (ab229449)

    Immunohistochemical analysis of paraffin embedded Human thyroid gland follicular carcinoma tissue labeling Thyroglobulin with unpurified ab156008 antibody at 1/250.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab156008).

  • Immunocytochemistry/ Immunofluorescence - Anti-Thyroglobulin antibody [EPR9730] - Low endotoxin, Azide free (ab229449)
    Immunocytochemistry/ Immunofluorescence - Anti-Thyroglobulin antibody [EPR9730] - Low endotoxin, Azide free (ab229449)

    Immunofluorescent analysis of TT cells labeling Thyroglobulin with unpurified ab156008 at 1/50.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab156008).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroglobulin antibody [EPR9730] - Low endotoxin, Azide free (ab229449)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroglobulin antibody [EPR9730] - Low endotoxin, Azide free (ab229449)

    This IHC data was generated using the same anti-Thyroglobulin antibody clone, EPR9730, in a different buffer formulation (cat# ab156008).

    Immunohistochemical analysis of paraffin embedded Human thyroid gland follicular carcinoma tissue labeling Thyroglobulin with unpurified ab156008 antibody at 1/250.

  • Immunocytochemistry/ Immunofluorescence - Anti-Thyroglobulin antibody [EPR9730] - Low endotoxin, Azide free (ab229449)
    Immunocytochemistry/ Immunofluorescence - Anti-Thyroglobulin antibody [EPR9730] - Low endotoxin, Azide free (ab229449)

    This ICC/IF data was generated using the same anti-Thyroglobulin antibody clone, EPR9730, in a different buffer formulation (cat# ab156008).

    Immunofluorescent analysis of TT cells labeling Thyroglobulin with unpurified ab156008 at 1/50.

  • Anti-Thyroglobulin antibody [EPR9730] - Low endotoxin, Azide free (ab229449)
    Anti-Thyroglobulin antibody [EPR9730] - Low endotoxin, Azide free (ab229449)

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