Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Thyroglobulin antibody [CPT-R35.3-31] (ab243093)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [CPT-R35.3-31] to Thyroglobulin
  • Suitable for: ELISA, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Thyroglobulin antibody [CPT-R35.3-31]
    See all Thyroglobulin primary antibodies

  • Description

    Rabbit monoclonal [CPT-R35.3-31] to Thyroglobulin

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human thyroid and thyroid cancer tissues. ELISA: Immunizing peptide.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    CPT-R35.3-31

  • Isotype

    IgG

  • Research areas

Images

  • ELISA - Anti-Thyroglobulin antibody [CPT-R35.3-31] (ab243093)
    ELISA - Anti-Thyroglobulin antibody [CPT-R35.3-31] (ab243093)

    50 ng CPTAC-39c (1000 ng/mL, 50μL per well) was coated onto 96-wells. Serial dilutions (0, 0.2, 0.9, 3.9, 15, 62, 250, 1000 ng/mL) of ab243093 (50μL) were incubated with CPTAC-39c in each well for 60 minutes by shaking. This was followed by adding goat anti-rabbit IgG, (H+L), phosphatase-conjugated secondary antibody (50μL) into each well and incubating for another 40 minutes. After washing, 50 μL of PNPP was added and incubated for 15 minutes without shaking for color development. OD was read at 405nm within 5 minutes.

    The secondary antibody was used at 1/2500 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroglobulin antibody [CPT-R35.3-31] (ab243093)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroglobulin antibody [CPT-R35.3-31] (ab243093)

    Immunohistochemical analysis of paraffin-embedded human thyroid tissue labeling Thyroglobulin with ab243093 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on human thyroid (PMID: 17381352) is observed. Counter stained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroglobulin antibody [CPT-R35.3-31] (ab243093)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroglobulin antibody [CPT-R35.3-31] (ab243093)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue labeling Thyroglobulin with ab243093 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining on human thyroid cancer (PMID: 3061922) is observed. Counter stained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroglobulin antibody [CPT-R35.3-31] (ab243093)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroglobulin antibody [CPT-R35.3-31] (ab243093)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling Thyroglobulin with ab243093 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Negative control: No staining on human pancreas (PMID: 10817499) is observed. Counter stained with hematoxylin.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Anti-Thyroglobulin antibody [CPT-R35.3-31] (ab243093)
    Anti-Thyroglobulin antibody [CPT-R35.3-31] (ab243093)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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