Anti-Thyroglobulin antibody [CPT-R35.3-31] (ab243093)
![Anti-Thyroglobulin antibody [CPT-R35.3-31] (ab243093)](https://www.abcam.com/ps/products/243/ab243093/Images/ab243093-4-243093ELISA1.jpg "Anti-Thyroglobulin antibody [CPT-R35.3-31] (ab243093)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [CPT-R35.3-31] to Thyroglobulin
- Suitable for: ELISA, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-Thyroglobulin antibody [CPT-R35.3-31]
See all Thyroglobulin primary antibodies -
Description
Rabbit monoclonal [CPT-R35.3-31] to Thyroglobulin -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human thyroid and thyroid cancer tissues. ELISA: Immunizing peptide.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
CPT-R35.3-31 -
Isotype
IgG -
Research areas
Images
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ELISA - Anti-Thyroglobulin antibody [CPT-R35.3-31] (ab243093)
50 ng CPTAC-39c (1000 ng/mL, 50μL per well) was coated onto 96-wells. Serial dilutions (0, 0.2, 0.9, 3.9, 15, 62, 250, 1000 ng/mL) of ab243093 (50μL) were incubated with CPTAC-39c in each well for 60 minutes by shaking. This was followed by adding goat anti-rabbit IgG, (H+L), phosphatase-conjugated secondary antibody (50μL) into each well and incubating for another 40 minutes. After washing, 50 μL of PNPP was added and incubated for 15 minutes without shaking for color development. OD was read at 405nm within 5 minutes.
The secondary antibody was used at 1/2500 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroglobulin antibody [CPT-R35.3-31] (ab243093)](https://www.abcam.com/ps/products/243/ab243093/Images/ab243093-1-243093IHC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroglobulin antibody [CPT-R35.3-31] (ab243093)Immunohistochemical analysis of paraffin-embedded human thyroid tissue labeling Thyroglobulin with ab243093 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on human thyroid (PMID: 17381352) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroglobulin antibody [CPT-R35.3-31] (ab243093)](https://www.abcam.com/ps/products/243/ab243093/Images/ab243093-2-243093IHC2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroglobulin antibody [CPT-R35.3-31] (ab243093)Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue labeling Thyroglobulin with ab243093 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining on human thyroid cancer (PMID: 3061922) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroglobulin antibody [CPT-R35.3-31] (ab243093)](https://www.abcam.com/ps/products/243/ab243093/Images/ab243093-3-243093IHC3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroglobulin antibody [CPT-R35.3-31] (ab243093)Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling Thyroglobulin with ab243093 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Negative control: No staining on human pancreas (PMID: 10817499) is observed. Counter stained with hematoxylin.
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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![Anti-Thyroglobulin antibody [CPT-R35.3-31] (ab243093)](https://www.abcam.com/ps/products/243/ab243093/Images/ab243093-5-benefits-of-recombinant-antibodies.png)
Anti-Thyroglobulin antibody [CPT-R35.3-31] (ab243093)
