Antibodies, Proteins, Kits and Reagents for Life Science

314 937 ₸ Product size

100 µg 314 937 ₸ 10 µg 107 212 ₸

Order now and get it on Tuesday March 09, 2021

Anti-IP3 receptor antibody [L24/18] (ab252536)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Mouse monoclonal [L24/18] to IP3 receptor
Suitable for: IHC-Fr, WB, IHC-P, Flow Cyt
Reacts with: Mouse, Rat, Human

Product name

Anti-IP3 receptor antibody [L24/18]
See all IP3 receptor primary antibodies
Description

Mouse monoclonal [L24/18] to IP3 receptor
Host species

Mouse

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-Fr	Mouse Rat
IHC-P	Mouse Rat Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa and HuT-78 whole cell lysates; mouse brain lysate; rat brain lysate. IHC-P: Rat cerebellum tissue; mouse cerebellum tissue; human cerebellum tissue. IHC-Fr: Mouse cerebellum tissue; rat cerebellum tissue. Flow cyt: SH-SY5Y cells.
General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

L24/18
Isotype

IgG1
Research areas

Flow Cytometry - Anti-IP3 receptor antibody [L24/18] (ab252536)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized SH-SY5Y cells labeling IP3 receptor with ab252536 at 10.825µg/ml (red) compared with a Isotype control details (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti mouse IgG (Alexa Fluor^® 488, ab150113), at 1/2000 dilution was used as the secondary antibody.
Western blot - Anti-IP3 receptor antibody [L24/18] (ab252536)

All lanes : Anti-IP3 receptor antibody [L24/18] (ab252536) at 1/1000 dilution

Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 2 : HuT-78 (human Sezary syndrome cutaneous T lymphocyte), whole cell lysate
Lane 3 : Mouse brain tissue lysate
Lane 4 : Rat brain tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG at 1/10000 dilution

Predicted band size: 314 kDa
Observed band size: 314 kDa

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 3.25 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3 receptor antibody [L24/18] (ab252536)

Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue labeling IP3 receptor with ab252536 at 0.108µg/ml, followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Positive staining on rat cerebellum is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

The section was incubated with ab252536 for 30 mins at RT.

The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3 receptor antibody [L24/18] (ab252536)

Immunohistochemical analysis of paraffin-embedded human cerebellum tissue labeling IP3 receptor with ab252536 at 0.108µg/ml, followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Positive staining on human cerebellum is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

The section was incubated with ab252536 for 30 mins at RT.

The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3 receptor antibody [L24/18] (ab252536)

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue labeling IP3 receptor with ab252536 at 0.108µg/ml, followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Positive staining on mouse cerebellum is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

The section was incubated with ab252536 for 30 mins at RT.

The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument.
Immunohistochemistry (Frozen sections) - Anti-IP3 receptor antibody [L24/18] (ab252536)

Immunohistochemical analysis of frozen section of 4%PFA-fixed, 0.2% Triton X-100 permeabilized mouse cerebellum tissue labeling IP3 receptor with ab252536 at 4.33µg/ml, followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (green). Positive staining on mouse cerebellum is observed. The nuclear counter stain is DAPI (blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody isb150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20.
Immunohistochemistry (Frozen sections) - Anti-IP3 receptor antibody [L24/18] (ab252536)

Immunohistochemical analysis of frozen section of 4%PFA-fixed, 0.2% Triton X-100 permeabilized rat cerebellum tissue labeling IP3 receptor with ab252536 at 4.33µg/ml, followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (green). Positive staining on rat cerebellum is observed. The nuclear counter stain is DAPI (blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody isb150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20.
Anti-IP3 receptor antibody [L24/18] (ab252536)

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