Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IP3 receptor antibody [L24/18] (ab252536)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [L24/18] to IP3 receptor
  • Suitable for: IHC-Fr, WB, IHC-P, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-IP3 receptor antibody [L24/18]
    See all IP3 receptor primary antibodies

  • Description

    Mouse monoclonal [L24/18] to IP3 receptor

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-Fr
    Mouse
    Rat
    IHC-P
    Mouse
    Rat
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa and HuT-78 whole cell lysates; mouse brain lysate; rat brain lysate. IHC-P: Rat cerebellum tissue; mouse cerebellum tissue; human cerebellum tissue. IHC-Fr: Mouse cerebellum tissue; rat cerebellum tissue. Flow cyt: SH-SY5Y cells.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Flow Cytometry - Anti-IP3 receptor antibody [L24/18] (ab252536)
    Flow Cytometry - Anti-IP3 receptor antibody [L24/18] (ab252536)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized SH-SY5Y cells labeling IP3 receptor with ab252536 at 10.825µg/ml (red) compared with a Isotype control details (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti mouse IgG (Alexa Fluor® 488, ab150113), at 1/2000 dilution was used as the secondary antibody.

  • Western blot - Anti-IP3 receptor antibody [L24/18] (ab252536)
    Western blot - Anti-IP3 receptor antibody [L24/18] (ab252536)
    All lanes : Anti-IP3 receptor antibody [L24/18] (ab252536) at 1/1000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 2 : HuT-78 (human Sezary syndrome cutaneous T lymphocyte), whole cell lysate
    Lane 3 : Mouse brain tissue lysate
    Lane 4 : Rat brain tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG at 1/10000 dilution

    Predicted band size: 314 kDa
    Observed band size: 314 kDa



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3.25 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3 receptor antibody [L24/18] (ab252536)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3 receptor antibody [L24/18] (ab252536)

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue labeling IP3 receptor with ab252536 at 0.108µg/ml, followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Positive staining on rat cerebellum is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    The section was incubated with ab252536 for 30 mins at RT.

    The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3 receptor antibody [L24/18] (ab252536)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3 receptor antibody [L24/18] (ab252536)

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue labeling IP3 receptor with ab252536 at 0.108µg/ml, followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Positive staining on human cerebellum is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    The section was incubated with ab252536 for 30 mins at RT.

    The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3 receptor antibody [L24/18] (ab252536)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3 receptor antibody [L24/18] (ab252536)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue labeling IP3 receptor with ab252536 at 0.108µg/ml, followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Positive staining on mouse cerebellum is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    The section was incubated with ab252536 for 30 mins at RT.

    The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.

  • Immunohistochemistry (Frozen sections) - Anti-IP3 receptor antibody [L24/18] (ab252536)
    Immunohistochemistry (Frozen sections) - Anti-IP3 receptor antibody [L24/18] (ab252536)

    Immunohistochemical analysis of frozen section of 4%PFA-fixed, 0.2% Triton X-100 permeabilized mouse cerebellum tissue labeling IP3 receptor with ab252536 at 4.33µg/ml, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (green). Positive staining on mouse cerebellum is observed. The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody isb150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20.

     

  • Immunohistochemistry (Frozen sections) - Anti-IP3 receptor antibody [L24/18] (ab252536)
    Immunohistochemistry (Frozen sections) - Anti-IP3 receptor antibody [L24/18] (ab252536)

    Immunohistochemical analysis of frozen section of 4%PFA-fixed, 0.2% Triton X-100 permeabilized rat cerebellum tissue labeling IP3 receptor with ab252536 at 4.33µg/ml, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (green). Positive staining on rat cerebellum is observed. The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody isb150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20.

  • Anti-IP3 receptor antibody [L24/18] (ab252536)
    Anti-IP3 receptor antibody [L24/18] (ab252536)

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