Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IP3 receptor antibody [EPR4536] - BSA and Azide free (ab248112)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4536] to IP3 receptor - BSA and Azide free
  • Suitable for: IHC-P, Flow Cyt, WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-IP3 receptor antibody [EPR4536] - BSA and Azide free
    See all IP3 receptor primary antibodies

  • Description

    Rabbit monoclonal [EPR4536] to IP3 receptor - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, Flow Cyt, WBmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab248112 is the carrier-free version of ab125076. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab248112 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-IP3 receptor antibody [EPR4536] - BSA and Azide free (ab248112)
    Western blot - Anti-IP3 receptor antibody [EPR4536] - BSA and Azide free (ab248112)

    This data was developed using ab125076, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 cell lysate (20 µg)

    Lane 2: IP3 receptor knockout HAP1 cell lysate (20 µg) 

    Lane 3: Human brain tissue lysate (20 µg)

    Lane 4: SH-SY5Y cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab125076 observed at 270 kDa. Red - loading control, ab18058, observed at 124 kDa.

    ab125076 was shown to specifically recognize IP3 receptor in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when IP3 receptor knockout samples were examined. Wild-type and IP3 receptor knockout samples were subjected to SDS-PAGE. ab125076 and ab18058 (loading control to Vinculin) were diluted 1/1000 and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3 receptor antibody [EPR4536] - BSA and Azide free (ab248112)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3 receptor antibody [EPR4536] - BSA and Azide free (ab248112)
    This data was developed using ab125076, the same antibody clone in a different buffer formulation.ab125076, at 1/50, staining ITPR1 in paraffin-embedded Human brain tissue by immunohistochemistry. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Western blot - Anti-IP3 receptor antibody [EPR4536] - BSA and Azide free (ab248112)
    Western blot - Anti-IP3 receptor antibody [EPR4536] - BSA and Azide free (ab248112)
    All lanes : Anti-IP3 receptor antibody [EPR4536] (ab125076) at 1/1000 dilution

    Lane 1 : SH-SY5Y cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : HuT78 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 314 kDa



    This data was developed using ab125076, the same antibody clone in a different buffer formulation.

     

  • Anti-IP3 receptor antibody [EPR4536] - BSA and Azide free (ab248112)
    Anti-IP3 receptor antibody [EPR4536] - BSA and Azide free (ab248112)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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