Anti-Alpha B Crystallin antibody (ab231268)
Key features and details
- Rabbit polyclonal to Alpha B Crystallin
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-Alpha B Crystallin antibody
See all Alpha B Crystallin primary antibodies -
Description
Rabbit polyclonal to Alpha B Crystallin -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human, Pig
Predicted to work with: Rat, Sheep, Rabbit, Cow, Cynomolgus monkey, Orangutan
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Immunogen
Recombinant full length protein (His-T7-tag) corresponding to Human Alpha B Crystallin aa 1 to the C-terminus. (Expressed in E.coli).
Database link: P02511 -
Positive control
- IHC-P: Human cerebrum, glioma and kidney tissues. WB: Mouse brain, heart and crebrum lysates; Pig brain and cerebrum lysates; Recombinant human Alpha B Crystallin protein.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab231268 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha B Crystallin antibody (ab231268)
Paraffin-embedded human cerebrum tissue stained for Alpha B Crystallin antibody using ab231268 at 10 µg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha B Crystallin antibody (ab231268)Paraffin-embedded human glioma tissue stained for Alpha B Crystallin antibody using ab231268 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha B Crystallin antibody (ab231268)Paraffin-embedded human kidney tissue stained for Alpha B Crystallin antibody using ab231268 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Western blot - Anti-Alpha B Crystallin antibody (ab231268)Anti-Alpha B Crystallin antibody (ab231268) at 2 µg/ml + Recombinant human Alpha B Crystallin protein.
Predicted band size: 20 kDa
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Western blot - Anti-Alpha B Crystallin antibody (ab231268)All lanes : Anti-Alpha B Crystallin antibody (ab231268) at 3 µg/ml
Lane 1 : Mouse brain lysate
Lane 2 : Mouse heart lysate
Lane 3 : Pig brain lysate
Secondary
All lanes : HRP-Linked Guinea pig Anti-Rabbit antibody at 1/2000 dilution
Predicted band size: 20 kDa
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Western blot - Anti-Alpha B Crystallin antibody (ab231268)Anti-Alpha B Crystallin antibody (ab231268) at 2 µg/ml + Pig cerebrum lysate.
Predicted band size: 20 kDa
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Western blot - Anti-Alpha B Crystallin antibody (ab231268)Anti-Alpha B Crystallin antibody (ab231268) at 2 µg/ml + Mouse cerebrum lysate.
Predicted band size: 20 kDa
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Western blot - Anti-Alpha B Crystallin antibody (ab231268)Anti-Alpha B Crystallin antibody (ab231268) at 2 µg/ml + Mouse heart lysate.
Predicted band size: 20 kDa
