Anti-AFM antibody [EPR19383] (ab201034)
![Anti-AFM antibody [EPR19383] (ab201034)](https://www.abcam.com/ps/products/201/ab201034/Images/ab201034-255663-anti-afm-antibody-epr19383-western-blot.jpg "Anti-AFM antibody [EPR19383] (ab201034)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19383] to AFM
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-AFM antibody [EPR19383]
See all AFM primary antibodies -
Description
Rabbit monoclonal [EPR19383] to AFM -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHuman
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Untreated and PNGase F treated mouse serum; Human serum, Human blood, mouse plasma, mouse serum, rat serum, rat plasma and Human plasma.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19383 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-AFM antibody [EPR19383] (ab201034)All lanes : Anti-AFM antibody [EPR19383] (ab201034) at 1/1000 dilution
Lane 1 : Human serum at 20 µg
Lane 2 : Human blood at 20 µg
Lane 3 : Mouse plasma at 20 µg
Lane 4 : Mouse serum at 20 µg
Lane 5 : Rat serum at 20 µg
Lane 6 : Rat plasma at 20 µg
Lane 7 : Human plasma at 10 µg
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 69 kDa
Observed band size: 87 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1,2,3,4,5 and 7: 30 seconds; Lane 6: 3 seconds.
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![Western blot - Anti-AFM antibody [EPR19383] (ab201034)](https://www.abcam.com/ps/products/201/ab201034/Images/ab201034-255664-anti-afm-antibody-epr19383-western-blot.jpg)
Western blot - Anti-AFM antibody [EPR19383] (ab201034)All lanes : Anti-AFM antibody [EPR19383] (ab201034) at 1/1000 dilution
Lane 1 : Untreated mouse serum
Lane 2 : Mouse serum PNGase F treated
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 69 kDa
Observed band size: 65,87 kDa why is the actual band size different from the predicted?
Exposure time: 5 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
AFM is a glycoprotein, its apparent molecular weight on SDS-PAGE is 87 kDa due to glycosylation. AFM has 4 potential sites for N-Glycosylation and N-glycanase treatment reduced the apparent MW to 65 kDa (PMID:7517938).
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![Anti-AFM antibody [EPR19383] (ab201034)](https://www.abcam.com/ps/products/201/ab201034/Images/ab201034-4-benefits-of-recombinant-antibodies.png)
Anti-AFM antibody [EPR19383] (ab201034)
