AFM positive control ChIP primer pair (ab269259)
Key features and details
- AFM positive control ChIP primer pair
- Suitable for: ChIP
Overview
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Product name
AFM positive control ChIP primer pair
See all AFM primary antibodies -
Description
AFM positive control ChIP primer pair -
Tested applications
Suitable for: ChIPmore details -
General notes
Positive control ChIP-qPCR 5' and 3' primers for AFM gene. Use with SYBR green.
We recommend these primers as a positive control (based on Abcam's testing) for the Histone H3 dimethyl K9 histone mark. They may also be useful for other histone marks.
500pmole of each oligo per unit (lyophilised). HPLC purified, desalted and lyophilised as a sodium salt.
Quantity provided is sufficient for approx. 200 reactions based on 2.5pmol of primer per reaction with a final concentration of 100nM in 25µl.
Please contact us after purchase if you require the sequence of the oligos.
Properties
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Form
Lyophilized -
Storage instructions
Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Concentration information loading... -
Clonality
Monoclonal -
Research areas
Images
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ChIP - AFM positive control ChIP primer pair (ab269259)
Chromatin was prepared from U2OS cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25µg of chromatin, 2µg of ab1220 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first Kb of the transcribed region.
