Antibodies, Proteins, Kits and Reagents for Life Science

335 040 ₸ Product size

100 µl 335 040 ₸ 10 µl 137 366 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Alpha B Crystallin antibody [EPR2752] (ab76467)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR2752] to Alpha B Crystallin
Suitable for: WB, IHC-P, ICC/IF, IP
Reacts with: Rat, Human

Product name

Anti-Alpha B Crystallin antibody [EPR2752]
See all Alpha B Crystallin primary antibodies
Description

Rabbit monoclonal [EPR2752] to Alpha B Crystallin
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Rat Human

See all applications and species data

Immunogen

Synthetic peptide within Human Alpha B Crystallin (C terminal). The exact sequence is proprietary.
Positive control
- Fetal heart and rat eyeball lysates; human heart muscle tissue.
General notes
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR2752
Isotype

IgG
Research areas

Western blot - Anti-Alpha B Crystallin antibody [EPR2752] (ab76467)

Anti-Alpha B Crystallin antibody [EPR2752] (ab76467) at 1/1000 dilution (purified) + Rat eyeball lysate at 10 µg

Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 20 kDa
Observed band size: 22 kDa
why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Immunocytochemistry/ Immunofluorescence - Anti-Alpha B Crystallin antibody [EPR2752] (ab76467)

Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labelling Alpha B Crystallin with purified ab76467 at 1/250. Cells were fixed with 4% PFA and permeabilized with 0.1% triton X-100. ab150077 Goat anti rabbit IgG (Alexa Fluor^® 488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha B Crystallin antibody [EPR2752] (ab76467)

Immunohistochemical staining of paraffin embedded human brain with purified ab76467 at a working dilution of 1 in 250. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.
Western blot - Anti-Alpha B Crystallin antibody [EPR2752] (ab76467)

Anti-Alpha B Crystallin antibody [EPR2752] (ab76467) at 1/1000 dilution (purified) + Human fetal heart tissue lysate at 10 µg/ml

Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 20 kDa
Observed band size: 22 kDa why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Immunocytochemistry/ Immunofluorescence - Anti-Alpha B Crystallin antibody [EPR2752] (ab76467)

Immunofluorescence staining of HeLa cells with purified ab76467 at a working dilution of 1 in 200, counter-stained with DAPI. The secondary antibody was Alexa Fluor^® 488 goat anti rabbit, used at a dilution of 1 in 200. The cells were fixed in 4% PFA.
Western blot - Anti-Alpha B Crystallin antibody [EPR2752] (ab76467)

ab76467 (purified) at 1/250 immunoprecipitating alpha B crystallin in human fetal heart (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha B Crystallin antibody [EPR2752] (ab76467)

Immunohistochemical staining of paraffin embedded human brain with unpurified ab76467 at a working dilution of 1 in 250. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha B Crystallin antibody [EPR2752] (ab76467)

Immunohistochemical staining of alpha B Crystallin in paraffin embedded human heart muscle with ab76467 at a 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Western blot - Anti-Alpha B Crystallin antibody [EPR2752] (ab76467)

Lane 1 : Anti-Alpha B Crystallin antibody [EPR2752] (ab76467) at 1/5000 dilution
Lane 2 : Anti-Alpha B Crystallin antibody [EPR2752] (ab76467) at 1/20000 dilution

Lane 1 : fetal heart lysate
Lane 2 : rat eyeball lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 20 kDa
Observed band size: 20 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Alpha B Crystallin antibody [EPR2752] (ab76467)

Immunofluorescence staining of HeLa cells with unpurified ab76467 at a working dilution of 1 in 200, counter-stained with DAPI. The secondary antibody was Alexa Fluor^® 488 goat anti rabbit, used at a dilution of 1 in 200. The cells were fixed in 4% PFA.
Immunoprecipitation - Anti-Alpha B Crystallin antibody [EPR2752] (ab76467)

ab76467 (unpurified) at 1/250 immunoprecipitating alpha B crystallin in human fetal heart (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Anti-Alpha B Crystallin antibody [EPR2752] (ab76467)

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