Formalin-fixed, paraffin-embedded Rat Heart Muscle tissue stained for Alpha B Crystallin using ab268037 at 2 μg/ml for 30 minutes at RT in immunohistochemical analysis.

Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes.

Anti-Alpha B Crystallin antibody [CPTC-CRYAB-1] (ab268037) at 2 µg/ml + HeLa cell lysate

Predicted band size: 20 kDa



Western blot HeLa cell lysate using Crystallin Alpha B Mouse Monoclonal Antibody (CPTC-CYRAB-1).

Formalin-fixed, paraffin-embedded human breast tissue stained for Alpha B Crystallin using ab268037 at 2 μg/ml for 30 minutes at RT in immunohistochemical analysis.

Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes.

Protein Array containing more than 19,000 full-length human proteins using ab268037. Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.