Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

50 µl 321 638 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Alpha B Crystallin antibody [6D11] (ab230722)

Key features and details

Mouse monoclonal [6D11] to Alpha B Crystallin
Suitable for: WB, IP, IHC-P, Flow Cyt, ICC/IF
Reacts with: Rat, Dog, Human, African green monkey
Isotype: IgG1

Product name

Anti-Alpha B Crystallin antibody [6D11]
See all Alpha B Crystallin primary antibodies
Description

Mouse monoclonal [6D11] to Alpha B Crystallin
Host species

Mouse

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human African green monkey
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant full length protein corresponding to Human Alpha B Crystallin aa 1 to the C-terminus. Produced in HEK-293T cells.
Database link: P02511

Run BLAST with Run BLAST with
Positive control
- WB: COS-7 and PC-12 whole cell lysate. IHC-P: Human thyroid carcinoma and heart tissue. ICC/IF: HT-29 cells. IP: DDK tagged CRYAB overexpression-transfected HEK-293T whole cell lysate. Flow Cyt: Overexpression plasmid-transfected HEK-293T cells.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol
Concentration information loading...
Purity

Protein A/G purified
Clonality

Monoclonal
Clone number

6D11
Isotype

IgG1
Research areas

Western blot - Anti-Alpha B Crystallin antibody [6D11] (ab230722)

All lanes : Anti-Alpha B Crystallin antibody [6D11] (ab230722) at 1/500 dilution

Lane 1 : pCMV6-ENTRY control-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : pCMV6-ENTRY Alpha B Crystallin-transfected HEK-293T whole cell lysate

Lysates/proteins at 5 µg per lane.

Developed using the ECL technique.

Predicted band size: 20 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Alpha B Crystallin antibody [6D11] (ab230722)

COS-7 (African green monkey kidney fibroblast-like cell line) cells stained for Alpha B Crystallin (green) using ab230722 at 1/50 dilution in ICC/IF.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha B Crystallin antibody [6D11] (ab230722)

Formalin-fixed, paraffin-embedded human heart tissue stained for Alpha B Cristallin using ab230722 at 10μg/ml in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha B Crystallin antibody [6D11] (ab230722)

Formalin-fixed, paraffin-embedded human thyroid carcinoma tissue stained for Alpha B Cristallin using ab230722 at 1/50 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-Alpha B Crystallin antibody [6D11] (ab230722)

HT-29 (human colorectal adenocarcinoma cell line) cells stained for Alpha B Crystallin (green) using ab230722 at 1/50 dilution in ICC/IF.
Western blot - Anti-Alpha B Crystallin antibody [6D11] (ab230722)

All lanes : Anti-Alpha B Crystallin antibody [6D11] (ab230722) at 1/500 dilution

Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : HT-29 (human colorectal adenocarcinoma cell line) whole cell lysate
Lane 4 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 5 : COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysate
Lane 6 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 7 : MDCK (canine kidney cell line) whole cell lysate
Lane 8 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 9 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate

Lysates/proteins at 35 µg per lane.

Developed using the ECL technique.

Predicted band size: 20 kDa
Immunoprecipitation - Anti-Alpha B Crystallin antibody [6D11] (ab230722)

KAT7 / Hbo1 / MYST2 was immunoprecipitated from 1mg of DDK tagged Alpha B Crystallin overexpression lysate at a 1:5 dilution with HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with 2μg ab230722..

Lane 1: IP without ab230722 in DDK tagged Alpha B Crystallin overexpression lysate at a 1:5 dilution with HEK-293T whole cell lysate (negative control)

Lane 2: ab230722 IP in DDK tagged Alpha B Crystallin overexpression lysate at a 1:5 dilution with HEK-293T whole cell lysate.
Flow Cytometry - Anti-Alpha B Crystallin antibody [6D11] (ab230722)

Flow cytometric analysis of Alpha B Crystallin overexpression plasmid-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell line labeling Alpha B Crystallin with 230722 at 1/100 dilution (red) compared with an empty vector control plasmid-transfected HEK-293T cells (blue).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com