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Anti-SPARCL1 antibody [EPR22615-277] - BSA and Azide free (ab256506)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22615-277] to SPARCL1 - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-SPARCL1 antibody [EPR22615-277] - BSA and Azide free
    See all SPARCL1 primary antibodies

  • Description

    Rabbit monoclonal [EPR22615-277] to SPARCL1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human cerebral cortex and tonsil tissue.

  • General notes

    Ab256506 is the carrier-free version of ab255597. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab256506 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARCL1 antibody [EPR22615-277] - BSA and Azide free (ab256506)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARCL1 antibody [EPR22615-277] - BSA and Azide free (ab256506)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling SPARCL1 with ab255597 at 1/1000 dilution (0.623 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in high endothelial venules of human tonsil (PMID: 8626806) is observed. The section was incubated with ab255597 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255597).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARCL1 antibody [EPR22615-277] - BSA and Azide free (ab256506)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARCL1 antibody [EPR22615-277] - BSA and Azide free (ab256506)

    Immunohistochemical analysis of paraffin-embedded human cerebral cortex tissue labeling SPARCL1 with ab255597 at 1/1000 dilution (0.623 μg/ml)  followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in human cerebral cortex (PMID: 18381651, 9199668). The section was incubated with ab255597 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255597).

  • Anti-SPARCL1 antibody [EPR22615-277] - BSA and Azide free (ab256506)
    Anti-SPARCL1 antibody [EPR22615-277] - BSA and Azide free (ab256506)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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