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Anti-Alpha B Crystallin antibody [1B6.1-3G4] (ab13496)

Key features and details

  • Mouse monoclonal [1B6.1-3G4] to Alpha B Crystallin
  • Suitable for: WB, Flow Cyt, IHC-P
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-Alpha B Crystallin antibody [1B6.1-3G4]
    See all Alpha B Crystallin primary antibodies

  • Description

    Mouse monoclonal [1B6.1-3G4] to Alpha B Crystallin

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    Full length native protein (purified) corresponding to Cow Alpha B Crystallin.

  • Positive control

    • Recombinant Bovine alpha B Crystallin Protein.

  • General notes

    This product was changed from ascites to tissue culture supernatant on 31st May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

Images

  • Western blot - Anti-Alpha B Crystallin antibody [1B6.1-3G4] (ab13496)
    Western blot - Anti-Alpha B Crystallin antibody [1B6.1-3G4] (ab13496)
    All lanes : Anti-Alpha B Crystallin antibody [1B6.1-3G4] (ab13496) at 1/1000 dilution

    Lane 1 : Molecular weight marker
    Lane 2 : alpha Crystallin

    Lane 3 : alpha A Crystallin

    Lane 4 : alpha B Crystallin
    Lane 5 : beta Crystallin

    Predicted band size: 21 kDa



    This image was generated using the ascites version of the product.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha B Crystallin antibody [1B6.1-3G4] (ab13496)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha B Crystallin antibody [1B6.1-3G4] (ab13496)

    Immunohistochemical analysis of human heart tissue using ab13496 at the concentration 10ug/ml.

    This image was generated using the ascites version of the product.

  • Flow Cytometry - Anti-Alpha B Crystallin antibody [1B6.1-3G4] (ab13496)
    Flow Cytometry - Anti-Alpha B Crystallin antibody [1B6.1-3G4] (ab13496)

    Overlay histogram showing SH-SY5Y cells stained with ab13496 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab13496, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    This image was generated using the ascites version of the product.

  • Western blot - Anti-Alpha B Crystallin antibody [1B6.1-3G4] (ab13496)
    Western blot - Anti-Alpha B Crystallin antibody [1B6.1-3G4] (ab13496)

    This image was generated using the ascites version of the product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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