Anti-Histone H1.4 (tri methyl K25, phospho S26) antibody (ab33231)
Key features and details
- Rabbit polyclonal to Histone H1.4 (tri methyl K25, phospho S26)
- Suitable for: ELISA
- Reacts with: Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-Histone H1.4 (tri methyl K25, phospho S26) antibody
See all Histone H1.4 primary antibodies -
Description
Rabbit polyclonal to Histone H1.4 (tri methyl K25, phospho S26)This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »
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Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ELISA Recombinant fragment
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Immunogen
Synthetic peptide corresponding to Human Histone H1.4 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab35913)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species ELISA Recombinant fragmentAll applications MouseHumanApplication Abreviews Notes ELISA Use at an assay dependent concentration.Notes ELISA
Use at an assay dependent concentration.Target
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Function
Histones H1 are necessary for the condensation of nucleosome chains into higher order structures. -
Sequence similarities
Belongs to the histone H1/H5 family.
Contains 1 H15 (linker histone H1/H5 globular) domain. -
Post-translational
modificationsAcetylated at Lys-26. Deacetylated at Lys-26 by SIRT1. -
Cellular localization
Nucleus. Chromosome. - Information by UniProt
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Database links
- Entrez Gene: 3008 Human
- Entrez Gene: 50709 Mouse
- Omim: 142220 Human
- SwissProt: P10412 Human
- SwissProt: P43274 Mouse
- Unigene: 248133 Human
- Unigene: 170587 Mouse
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Alternative names
- H1 histone family member 4 antibody
- H1.4 antibody
- H14_HUMAN antibody
see all
Images
This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.-
ELISA - Anti-Histone H1.4 (tri methyl K25, phospho S26) antibody (ab33231)
This antibody gave a positive result in ELISA against the immunizing peptide, Human Histone H1.4 (tri methyl K25, phospho S26) peptide (blue line).
For the following peptides it gave following results:
the non-modified equivalent peptide (yellow line, ab35918) - weak result,
H1.4 phospho S26 peptide (pink line, ab35917) - weak result.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (1)
ab33231 has been referenced in 1 publication.
- Terme JM et al. Dynamics and dispensability of variant-specific histone H1 Lys-26/Ser-27 and Thr-165 post-translational modifications. FEBS Lett 588:2353-62 (2014). PubMed: 24873882
Images
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ELISA - Anti-Histone H1.4 (tri methyl K25, phospho S26) antibody (ab33231)
This antibody gave a positive result in ELISA against the immunizing peptide, Human Histone H1.4 (tri methyl K25, phospho S26) peptide (blue line).
For the following peptides it gave following results:
the non-modified equivalent peptide (yellow line, ab35918) - weak result,
H1.4 phospho S26 peptide (pink line, ab35917) - weak result.
