Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Histone H1.4 (tri methyl K25, phospho S26) antibody (ab33231)

Key features and details

  • Rabbit polyclonal to Histone H1.4 (tri methyl K25, phospho S26)
  • Suitable for: ELISA
  • Reacts with: Recombinant fragment
  • Isotype: IgG

Overview

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ELISA
Recombinant fragment
All applications
Mouse
Human
Application Abreviews Notes
ELISA
Use at an assay dependent concentration.
Notes
ELISA
Use at an assay dependent concentration.

Target

  • Function

    Histones H1 are necessary for the condensation of nucleosome chains into higher order structures.

  • Sequence similarities

    Belongs to the histone H1/H5 family.
    Contains 1 H15 (linker histone H1/H5 globular) domain.

  • Post-translational
    modifications

    Acetylated at Lys-26. Deacetylated at Lys-26 by SIRT1.

  • Cellular localization

    Nucleus. Chromosome.
  • Information by UniProt

  • Database links

    • Alternative names

      • H1 histone family member 4 antibody
      • H1.4 antibody
      • H14_HUMAN antibody
      • H1E antibody
      • H1F4 antibody
      • Hist1h1e antibody
      • Histone 1 H1e antibody
      • Histone cluster 1 H1e antibody
      • Histone H1 antibody
      • Histone H1.4 antibody
      • Histone H1B antibody
      • MGC116819 antibody
      see all

    Images

    This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

    • ELISA - Anti-Histone H1.4 (tri methyl K25, phospho S26) antibody (ab33231)
      ELISA - Anti-Histone H1.4 (tri methyl K25, phospho S26) antibody (ab33231)

      This antibody gave a positive result in ELISA against the immunizing peptide, Human Histone H1.4 (tri methyl K25, phospho S26) peptide (blue line).
      For the following peptides it gave following results:
      the non-modified equivalent peptide (yellow line, ab35918) - weak result,
      H1.4 phospho S26 peptide (pink line, ab35917) - weak result.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (1)

    Publishing research using ab33231? Please let us know so that we can cite the reference in this datasheet.

    ab33231 has been referenced in 1 publication.

    • Terme JM  et al. Dynamics and dispensability of variant-specific histone H1 Lys-26/Ser-27 and Thr-165 post-translational modifications. FEBS Lett 588:2353-62 (2014). PubMed: 24873882

    Images

    • ELISA - Anti-Histone H1.4 (tri methyl K25, phospho S26) antibody (ab33231)
      ELISA - Anti-Histone H1.4 (tri methyl K25, phospho S26) antibody (ab33231)

      This antibody gave a positive result in ELISA against the immunizing peptide, Human Histone H1.4 (tri methyl K25, phospho S26) peptide (blue line).
      For the following peptides it gave following results:
      the non-modified equivalent peptide (yellow line, ab35918) - weak result,
      H1.4 phospho S26 peptide (pink line, ab35917) - weak result.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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