Antibodies, Proteins, Kits and Reagents for Life Science

154 118 ₸ Product size

100 µl 154 118 ₸

Order now and get it on Tuesday March 09, 2021

Anti-RAB29 antibody [MJF-R30-104] (ab256527)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [MJF-R30-104] to RAB29
Suitable for: WB, IP
Knockout validated
Reacts with: Human

Product name

Anti-RAB29 antibody [MJF-R30-104]
See all RAB29 primary antibodies
Description

Rabbit monoclonal [MJF-R30-104] to RAB29
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Human

WB
Human

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Wild type A549, A549, HEK293T cells and HEK293T cells lysates. IP: A549 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IP	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

MJF-R30-104
Isotype

IgG
Research areas

Western blot - Anti-RAB29 antibody [EPRMJF-30-104] (ab256527)

All lanes : Anti-RAB29 antibody [MJF-R30-104] (ab256527) at 1/4000 dilution

Lane 1 : HEK293T cells (human embryonic kidney epithelial cell) transiently transfected with non-target siRNA control whole cell lysate 15ug
Lane 2 : HEK293T cells (human embryonic kidney epithelial cell) transiently transfected with siRNA against Rab29 whole cell lysate 15ug

Secondary
All lanes : IR Dye 800CW Goat anti-rabbit IgG (#926-32211) at 1/10000 dilution

Predicted band size: 23 kDa
Observed band size: 23 kDa

This image was kindly provided by Dr. Mark Cookson, NIH

Blocking/Diluting buffer and concentration: 50% TBST, 50% Odyssey Blocking buffer
Western blot - Anti-RAB29 antibody [EPRMJF-30-104] (ab256527)

All lanes : Anti-RAB29 antibody [MJF-R30-104] (ab256527) at 1/1000 dilution

Lane 1 : Wild type A549 (human lung carcinoma epithelial cell) whole cell lysate 20ug
Lane 2 : A549 (human lung carcinoma epithelial cell) Rab29 KO whole cell lysate 20ug

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 23 kDa
Observed band size: 23 kDa

The lysates were kindly provided by Dr. Dario Alessi, University of Dundee.

Blocking/Diluting buffer and concentration: 5% NFDM/TBST

Exposure Time: 59 seconds
Immunoprecipitation - Anti-RAB29 antibody [EPRMJF-30-104] (ab256527)

RAB29 was immunoprecipitated from 0.35 mg A549 (human lung carcinoma epithelial cell) whole cell lysate with ab256527 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab256527 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1: A549 (human lung carcinoma epithelial cell) whole cell lysate 10ug

Lane 2: ab256527 IP in A549 whole cell lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab256527 in A549 whole cell lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST

Exposure time: 30 seconds
Anti-RAB29 antibody [MJF-R30-104] (ab256527)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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