Anti-RAB29 antibody [MJF-R30-104] - BSA and Azide free (ab256548)
![Anti-RAB29 antibody [MJF-R30-104] - BSA and Azide free (ab256548)](https://www.abcam.com/ps/products/256/ab256548/Images/ab256548-353832-antirab29antibodymjfr30104westernblota549kohuman.jpg "Anti-RAB29 antibody [MJF-R30-104] - BSA and Azide free (ab256548)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [MJF-R30-104] to RAB29 - BSA and Azide free
- Suitable for: WB, IP
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-RAB29 antibody [MJF-R30-104] - BSA and Azide free
See all RAB29 primary antibodies -
Description
Rabbit monoclonal [MJF-R30-104] to RAB29 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Wild type A549, A549, HEK293T cells and HEK293T cells lysates. IP: A549 cells.
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General notes
ab256548 is a PBS-only buffer format of ab256527. Please refer to ab256527 for recommended dilutions, protocols, and image data.
The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®).
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
MJF-R30-104 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-RAB29 antibody [MJF-R30-104] - BSA and Azide free (ab256548)All lanes : Anti-RAB29 antibody [MJF-R30-104] (ab256527) at 1/1000 dilution
Lane 1 : Wild type A549 (human lung carcinoma epithelial cell) whole cell lysate
Lane 2 : A549 (human lung carcinoma epithelial cell) Rab29 KO whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 23 kDa
Observed band size: 23 kDaThe lysates were kindly provided by Dr. Dario Alessi, University of Dundee.
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Exposure Time: 59 seconds
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256527).
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![Immunoprecipitation - Anti-RAB29 antibody [MJF-R30-104] - BSA and Azide free (ab256548)](https://www.abcam.com/ps/products/256/ab256548/Images/ab256548-1-anti-rab29-antibody-mjfr30-104-immunoprecipitation-a549-human.jpg)
Immunoprecipitation - Anti-RAB29 antibody [MJF-R30-104] - BSA and Azide free (ab256548)RAB29 was immunoprecipitated from 0.35 mg A549 (human lung carcinoma epithelial cell) whole cell lysate with ab256527 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab256527 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.
Lane 1: A549 (human lung carcinoma epithelial cell) whole cell lysate 10ug
Lane 2: ab256527 IP in A549 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab256527 in A549 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST
Exposure time: 30 seconds
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256527).
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![Anti-RAB29 antibody [MJF-R30-104] - BSA and Azide free (ab256548)](https://www.abcam.com/ps/products/256/ab256548/Images/ab256548-3-benefits-of-recombinant-antibodies.png)
Anti-RAB29 antibody [MJF-R30-104] - BSA and Azide free (ab256548)
