Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Fragilis antibody [EPR5242] - BSA and Azide free (ab239978)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5242] to Fragilis - BSA and Azide free
  • Suitable for: IHC-P, ICC/IF, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Fragilis antibody [EPR5242] - BSA and Azide free
    See all Fragilis primary antibodies

  • Description

    Rabbit monoclonal [EPR5242] to Fragilis - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ICC/IF, WBmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab239978 is the carrier-free version of ab109429 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab239978 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fragilis antibody [EPR5242] - BSA and Azide free (ab239978)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fragilis antibody [EPR5242] - BSA and Azide free (ab239978)

    Immunohistochemical analysis of Fragilis in paraffin-embedded Human kidney tissue using ab109429 at 1/250 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109429).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Fragilis antibody [EPR5242] - BSA and Azide free (ab239978)
    Immunocytochemistry/ Immunofluorescence - Anti-Fragilis antibody [EPR5242] - BSA and Azide free (ab239978)

    ICC/IF image of ab109429 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab109429 at 1/50 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109429).

  • Anti-Fragilis antibody [EPR5242] - BSA and Azide free (ab239978)
    Anti-Fragilis antibody [EPR5242] - BSA and Azide free (ab239978)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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