Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Fragilis antibody (ab15592)

Key features and details

  • Rabbit polyclonal to Fragilis
  • Suitable for: IP, ICC/IF, IHC-Fr, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-Fragilis antibody
    See all Fragilis primary antibodies

  • Description

    Rabbit polyclonal to Fragilis

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, ICC/IF, IHC-Fr, WBmore details

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat

  • Immunogen

    Synthetic peptide corresponding to Mouse Fragilis aa 1-100 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab15737)

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab15592 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
Use at an assay dependent concentration.
ICC/IF (1)
Use a concentration of 5 µg/ml.
IHC-Fr
Use a concentration of 10 µg/ml.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 13 kDa (predicted molecular weight: 15 kDa).
Notes
IP
Use at an assay dependent concentration.
ICC/IF
Use a concentration of 5 µg/ml.
IHC-Fr
Use a concentration of 10 µg/ml.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 13 kDa (predicted molecular weight: 15 kDa).

Target

  • Function

    IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus (WNV), by inhibiting the early step(s) of replication.

  • Sequence similarities

    Belongs to the CD225 family.

  • Post-translational
    modifications

    Palmitoylation on membrane-proximal cysteines controls clustering in membrane compartments and antiviral activity against influenza virus.

  • Cellular localization

    Cell membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 1 8U antibody
    • Fragilis antibody
    • IFITM3 antibody
    • IFM3_HUMAN antibody
    • Interferon induced transmembrane protein 3 (1 8U) antibody
    • Interferon Induced Transmembrane Protein 3 antibody
    • Interferon inducible antibody
    • Interferon inducible protein 1 8U antibody
    • Interferon Inducible Protein 15 antibody
    • Interferon Inducible Protein Homolog antibody
    • Interferon-induced transmembrane protein 3 antibody
    • Interferon-inducible protein 1-8U antibody
    • IP15 antibody
    see all

Images

  • Western blot - Anti-Fragilis antibody (ab15592)
    Western blot - Anti-Fragilis antibody (ab15592)
    All lanes : Anti-Fragilis antibody (ab15592) at 1 µg/ml

    Lane 1 : Mouse Embryonic Stem Cell lysate
    Lane 2 : Mouse Embryonic Germ Cell lysate
    Lane 3 : Mouse Embryonic Stem Cell lysate with Fragilis peptide (ab15737) at 1 µg/ml
    Lane 4 : Mouse Embryonic Germ Cell lysate with Fragilis peptide (ab15737) at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 15 kDa

  • Immunohistochemistry (Frozen sections) - Anti-Fragilis antibody (ab15592)
    Immunohistochemistry (Frozen sections) - Anti-Fragilis antibody (ab15592)This image is courtesy of Petra Hajkova, University of Cambridge

    IHC showing localisation of Fragilis (red) in cryosections of male genital ridges of E12.5 mouse embryos. Germ cells can be identified by staining with anti-Oct4 (green).

    ab15592 (anti-Fragilis) = red
    Oct4 = green
    DAPI (DNA) = blue

    The dilution of ab15592 used was 10 ug/ml.

  • Immunocytochemistry/ Immunofluorescence - Anti-Fragilis antibody (ab15592)
    Immunocytochemistry/ Immunofluorescence - Anti-Fragilis antibody (ab15592)
    Anti-Fragilis antibody, ab15592, recognised a small proportion of cells in a culture of human embryonic stem cells differentiated into a heterogenous mix of cells. Based upon the sequence of the immunogen used to generate this antibody the protein it recognizes is likely to be IFITM2 and/or IFITM3.
  • Immunoprecipitation - Anti-Fragilis antibody (ab15592)
    Immunoprecipitation - Anti-Fragilis antibody (ab15592)
    Fragilis - Primordial Germ Cell Marker was immunoprecipitated using 0.5mg E14Tg2a whole cell extract, 5µg of Rabbit polyclonal to Fragilis and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, E14Tg2a whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab15592.
    Secondary: Clean blot (HRP conjugate) at 1/1000 dilution.
    Band: 13kDa: Fragilis - Primordial Germ Cell Marker.

References (25)

Publishing research using ab15592? Please let us know so that we can cite the reference in this datasheet.

ab15592 has been referenced in 25 publications.

  • Ahi YS  et al. IFITM3 Reduces Retroviral Envelope Abundance and Function and Is Counteracted by glycoGag. mBio 11:N/A (2020). PubMed: 31964738
  • Wu M  et al. Resveratrol alleviates chemotherapy-induced oogonial stem cell apoptosis and ovarian aging in mice. Aging (Albany NY) 11:1030-1044 (2019). PubMed: 30779707
  • Gan CP  et al. IFITM3 knockdown reduces the expression of CCND1 and CDK4 and suppresses the growth of oral squamous cell carcinoma cells. Cell Oncol (Dordr) N/A:N/A (2019). PubMed: 30949979
  • Fan X  et al. Single-cell reconstruction of follicular remodeling in the human adult ovary. Nat Commun 10:3164 (2019). PubMed: 31320652
  • Hou L  et al. Characteristics of Female Germline Stem Cells from Porcine Ovaries at Sexual Maturity. Cell Transplant 27:1195-1202 (2018). PubMed: 29991280
View all Publications for this product

Images

  • Western blot - Anti-Fragilis antibody (ab15592)
    Western blot - Anti-Fragilis antibody (ab15592)
    All lanes : Anti-Fragilis antibody (ab15592) at 1 µg/ml

    Lane 1 : Mouse Embryonic Stem Cell lysate
    Lane 2 : Mouse Embryonic Germ Cell lysate
    Lane 3 : Mouse Embryonic Stem Cell lysate with Fragilis peptide (ab15737) at 1 µg/ml
    Lane 4 : Mouse Embryonic Germ Cell lysate with Fragilis peptide (ab15737) at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 15 kDa

  • Immunohistochemistry (Frozen sections) - Anti-Fragilis antibody (ab15592)
    Immunohistochemistry (Frozen sections) - Anti-Fragilis antibody (ab15592) This image is courtesy of Petra Hajkova, University of Cambridge

    IHC showing localisation of Fragilis (red) in cryosections of male genital ridges of E12.5 mouse embryos. Germ cells can be identified by staining with anti-Oct4 (green).

    ab15592 (anti-Fragilis) = red
    Oct4 = green
    DAPI (DNA) = blue

    The dilution of ab15592 used was 10 ug/ml.

  • Immunocytochemistry/ Immunofluorescence - Anti-Fragilis antibody (ab15592)
    Immunocytochemistry/ Immunofluorescence - Anti-Fragilis antibody (ab15592)
    Anti-Fragilis antibody, ab15592, recognised a small proportion of cells in a culture of human embryonic stem cells differentiated into a heterogenous mix of cells. Based upon the sequence of the immunogen used to generate this antibody the protein it recognizes is likely to be IFITM2 and/or IFITM3.
  • Immunoprecipitation - Anti-Fragilis antibody (ab15592)
    Immunoprecipitation - Anti-Fragilis antibody (ab15592)
    Fragilis - Primordial Germ Cell Marker was immunoprecipitated using 0.5mg E14Tg2a whole cell extract, 5µg of Rabbit polyclonal to Fragilis and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, E14Tg2a whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab15592.
    Secondary: Clean blot (HRP conjugate) at 1/1000 dilution.
    Band: 13kDa: Fragilis - Primordial Germ Cell Marker.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-Fragilis antibody (ab15592)

Sign up