Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Jagged1 antibody [EPR4290] (ab109536)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4290] to Jagged1
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Jagged1 antibody [EPR4290]
    See all Jagged1 primary antibodies

  • Description

    Rabbit monoclonal [EPR4290] to Jagged1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HepG2 and NIH:OVCAR-3 cell lysate. Bladder cancer tissue. HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Jagged1 antibody [EPR4290] (ab109536)
    Western blot - Anti-Jagged1 antibody [EPR4290] (ab109536)
    All lanes : Anti-Jagged1 antibody [EPR4290] (ab109536) at 1/10000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : JAG1 knockout HAP1 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 134 kDa
    Observed band size: 180 kDa
    why is the actual band size different from the predicted?



    Lanes 1 - 2: Merged signal (red and green). Green - ab109536 observed at 180 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab109536 was shown to recognize in wild-type HAP1 cells as signal was lost at the expected MW in JAG1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and JAG1 knockout samples were subjected to SDS-PAGE. Ab109536 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Jagged1 antibody [EPR4290] (ab109536)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Jagged1 antibody [EPR4290] (ab109536)

    Immunohistochemical staining of paraffin embedded human kidney using purified ab109536 at a dilution of 1/300. A pre-diluted HRP polymer for rabbit IgG was used as the secondary and the sample was counter stained with hematoxylin. 

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-Jagged1 antibody [EPR4290] (ab109536)
    Western blot - Anti-Jagged1 antibody [EPR4290] (ab109536)
    Anti-Jagged1 antibody [EPR4290] (ab109536) at 1/1000 dilution (Purified) + HepG2 at 10 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 134 kDa
    Observed band size: 180 kDa why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-Jagged1 antibody [EPR4290] (ab109536)
    Western blot - Anti-Jagged1 antibody [EPR4290] (ab109536)
    All lanes : Anti-Jagged1 antibody [EPR4290] (ab109536) at 1/10000 dilution (Unpurified)

    Lane 1 : HepG2 cell lysate
    Lane 2 : NIH:OVCAR-3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 134 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Jagged1 antibody [EPR4290] (ab109536)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Jagged1 antibody [EPR4290] (ab109536)

    Immunohistochemical staining of Jagged1 in paraffin-embedded bladder cancer tissue using unpurified ab109536 at a dilution of 1/250.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-Jagged1 antibody [EPR4290] (ab109536)
    Anti-Jagged1 antibody [EPR4290] (ab109536)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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