Anti-ATP5A antibody [7H10BD4F9] (ab110273)
![Anti-ATP5A antibody [7H10BD4F9] (ab110273)](https://www.abcam.com/ps/products/110/ab110273/Images/ab110273-123468-anti-atp5a-antibody-7h10bd4f9-western-blot.gif "Anti-ATP5A antibody [7H10BD4F9] (ab110273)")
Key features and details
- Mouse monoclonal [7H10BD4F9] to ATP5A
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgG2b
Overview
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Product name
Anti-ATP5A antibody [7H10BD4F9]
See all ATP5A primary antibodies -
Description
Mouse monoclonal [7H10BD4F9] to ATP5A -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanWB Human
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Immunogen
Full length protein corresponding to Cow ATP5A.
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Positive control
- Isolated mitochondria from Human, Rat, Bovine and Mouse Heart, and HepG2, Cultured Human embryonic lung-derived fibroblasts (strain MRC5), HL-60 cells
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General notes
This antibody clone is manufactured by Abcam.
Product was previously marketed under the MitoSciences sub-brand.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
Purity
IgG fraction -
Purification notes
Near homogeneity as judged by SDS-PAGE. ab110273 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
Clonality
Monoclonal -
Clone number
7H10BD4F9 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-ATP5A antibody [7H10BD4F9] (ab110273)All lanes : Anti-ATP5A antibody [7H10BD4F9] (ab110273) at 1 µg/ml
Lane 1 : Human heart mitochondria at 5 µg
Lane 2 : Bovine heart mitochondria at 1 µg
Lane 3 : Rat heart mitochondria at 10 µg
Lane 4 : Mouse heart mitochondria at 10 µg
Lane 5 : HepG2 mitochondria at 20 µg
Predicted band size: 60 kDa
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![Flow Cytometry - Anti-ATP5A antibody [7H10BD4F9] (ab110273)](https://www.abcam.com/ps/products/110/ab110273/Images/ab110273-123467-anti-atp5a-antibody-7h10bd4f9-flow-cytometry.jpg)
Flow Cytometry - Anti-ATP5A antibody [7H10BD4F9] (ab110273)HL-60 cells were stained with 1 µg/mL ab110273 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry. -
![Immunocytochemistry/ Immunofluorescence - Anti-ATP5A antibody [7H10BD4F9] (ab110273)](https://www.abcam.com/ps/products/110/ab110273/Images/ab110273-123466-anti-atp5a-antibody-7h10bd4f9-immunofluorescence.gif)
Immunocytochemistry/ Immunofluorescence - Anti-ATP5A antibody [7H10BD4F9] (ab110273)Mitochondrial localization of ATP5A. Cultured Human embryonic lung-derived fibroblasts (strain MRC5) were fixed, permeabilized and then labeled with ab110273 (2 µg/ml) followed by an Alexa Fluor® 594-conjugated-goat-anti-mouse IgG2b isotype specific secondary antibody (2 µg/ml).
