Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-BIG1 antibody [EPR10046(2)] (ab183747)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10046(2)] to BIG1
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-BIG1 antibody [EPR10046(2)]
    See all BIG1 primary antibodies

  • Description

    Rabbit monoclonal [EPR10046(2)] to BIG1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Jurkat, 293T and A431 cell lysates; Human kidney tissue; 293T and A431 cells

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-BIG1 antibody [EPR10046(2)] (ab183747)
    Western blot - Anti-BIG1 antibody [EPR10046(2)] (ab183747)
    All lanes : Anti-BIG1 antibody [EPR10046(2)] (ab183747) at 1/1000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : A431 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution

    Predicted band size: 209 kDa
    Observed band size: 250 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIG1 antibody [EPR10046(2)] (ab183747)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIG1 antibody [EPR10046(2)] (ab183747)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling BIG1 with ab183747 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-BIG1 antibody [EPR10046(2)] (ab183747)
    Immunocytochemistry/ Immunofluorescence - Anti-BIG1 antibody [EPR10046(2)] (ab183747)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed 293T cells labeling BIG1 with ab183747 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-BIG1 antibody [EPR10046(2)] (ab183747)
    Immunocytochemistry/ Immunofluorescence - Anti-BIG1 antibody [EPR10046(2)] (ab183747)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed A431 cells labeling BIG1 with ab183747 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).

  • Anti-BIG1 antibody [EPR10046(2)] (ab183747)
    Anti-BIG1 antibody [EPR10046(2)] (ab183747)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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