Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-S1P1/EDG1 antibody [EPR21202] - BSA and Azide free (ab242085)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21202] to S1P1/EDG1 - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-S1P1/EDG1 antibody [EPR21202] - BSA and Azide free
    See all S1P1/EDG1 primary antibodies

  • Description

    Rabbit monoclonal [EPR21202] to S1P1/EDG1 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    WB is not recommended for mouse samples.

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human colon, diffuse large B-cell lymphoma and kidney tissue; Mouse kidney, cerebral cortex and spleen tissue.

  • General notes

    Ab242085 is the carrier-free version of ab233386. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab242085 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S1P1/EDG1 antibody [EPR21202] - BSA and Azide free (ab242085)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S1P1/EDG1 antibody [EPR21202] - BSA and Azide free (ab242085)

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling S1P1/EDG1 with ab233386 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous and cytoplasmic staining in endothelial cells of human colon (PMID:25588843) is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab233386).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S1P1/EDG1 antibody [EPR21202] - BSA and Azide free (ab242085)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S1P1/EDG1 antibody [EPR21202] - BSA and Azide free (ab242085)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling S1P1/EDG1 with ab233386 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous and cytoplasmic staining in endothelial cells of human kidney (PMID:25588843) is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab233386).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S1P1/EDG1 antibody [EPR21202] - BSA and Azide free (ab242085)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S1P1/EDG1 antibody [EPR21202] - BSA and Azide free (ab242085)

    Immunohistochemical analysis of paraffin-embedded human diffuse large B-cell lymphoma tissue labeling S1P1/EDG1 with ab233386 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous and cytoplasmic in endothelial cells and tumor cells of human diffuse large B-cell lymphoma (PMID: 22745305) is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab233386).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S1P1/EDG1 antibody [EPR21202] - BSA and Azide free (ab242085)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S1P1/EDG1 antibody [EPR21202] - BSA and Azide free (ab242085)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling S1P1/EDG1 with ab233386 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous and cytoplasmic in endothelial cells of mouse kidney (PMID: 25588843) is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab233386).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S1P1/EDG1 antibody [EPR21202] - BSA and Azide free (ab242085)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S1P1/EDG1 antibody [EPR21202] - BSA and Azide free (ab242085)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling S1P1/EDG1 with ab233386 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous and cytoplasmic in endothelial cells of mouse spleen(PMID: 25588843) is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab233386).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S1P1/EDG1 antibody [EPR21202] - BSA and Azide free (ab242085)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S1P1/EDG1 antibody [EPR21202] - BSA and Azide free (ab242085)

    Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue labeling S1P1/EDG1 with ab233386 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous and cytoplasmic in endothelial cells of mouse cerebral cortex (PMID: 25588843) is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab233386).

  • Anti-S1P1/EDG1 antibody [EPR21202] - BSA and Azide free (ab242085)
    Anti-S1P1/EDG1 antibody [EPR21202] - BSA and Azide free (ab242085)

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