Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-S1P1/EDG1 antibody [EPR21202] (ab233386)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21202] to S1P1/EDG1
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-S1P1/EDG1 antibody [EPR21202]
    See all S1P1/EDG1 primary antibodies

  • Description

    Rabbit monoclonal [EPR21202] to S1P1/EDG1

  • Host species

    Rabbit

  • Specificity

    WB is not recommended for mouse samples.

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Jurkat and BxPC-3 whole cell lysates. IHC-P: Human colon, diffuse large B-cell lymphoma and kidney tissue; Mouse kidney, cerebral cortex and spleen tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S1P1/EDG1 antibody [EPR21202] (ab233386)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S1P1/EDG1 antibody [EPR21202] (ab233386)

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling S1P1/EDG1 with ab233386 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous and cytoplasmic staining in endothelial cells of human colon (PMID:25588843) is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S1P1/EDG1 antibody [EPR21202] (ab233386)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S1P1/EDG1 antibody [EPR21202] (ab233386)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling S1P1/EDG1 with ab233386 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous and cytoplasmic staining in endothelial cells of human kidney (PMID:25588843) is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S1P1/EDG1 antibody [EPR21202] (ab233386)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S1P1/EDG1 antibody [EPR21202] (ab233386)

    Immunohistochemical analysis of paraffin-embedded human diffuse large B-cell lymphoma tissue labeling S1P1/EDG1 with ab233386 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous and cytoplasmic in endothelial cells and tumor cells of human diffuse large B-cell lymphoma (PMID: 22745305) is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Western blot - Anti-S1P1/EDG1 antibody [EPR21202] (ab233386)
    Western blot - Anti-S1P1/EDG1 antibody [EPR21202] (ab233386)
    All lanes : Anti-S1P1/EDG1 antibody [EPR21202] (ab233386) at 1/1000 dilution

    Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood), whole cell lysate
    Lane 2 : BxPC-3 (human pancreas adenocarcinoma cell line), whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 43 kDa
    Observed band size: 43 kDa


    Exposure time: 114 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    To avoid protein aggregation, the lysates were not boiled.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S1P1/EDG1 antibody [EPR21202] (ab233386)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S1P1/EDG1 antibody [EPR21202] (ab233386)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling S1P1/EDG1 with ab233386 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous and cytoplasmic in endothelial cells of mouse kidney (PMID: 25588843) is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S1P1/EDG1 antibody [EPR21202] (ab233386)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S1P1/EDG1 antibody [EPR21202] (ab233386)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling S1P1/EDG1 with ab233386 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous and cytoplasmic in endothelial cells of mouse spleen(PMID: 25588843) is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S1P1/EDG1 antibody [EPR21202] (ab233386)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S1P1/EDG1 antibody [EPR21202] (ab233386)

    Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue labeling S1P1/EDG1 with ab233386 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous and cytoplasmic in endothelial cells of mouse cerebral cortex (PMID: 25588843) is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Anti-S1P1/EDG1 antibody [EPR21202] (ab233386)
    Anti-S1P1/EDG1 antibody [EPR21202] (ab233386)

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