Anti-S1P1/EDG1 antibody (ab11424)
Key features and details
- Rabbit polyclonal to S1P1/EDG1
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-S1P1/EDG1 antibody
See all S1P1/EDG1 primary antibodies -
Description
Rabbit polyclonal to S1P1/EDG1 -
Host species
Rabbit -
Specificity
ab11424 detects S1P1/EDG1 protein in transfected human cell samples. This antibody shows no cross-reactivity to S1P3. -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Horse, Cow, Cat, Monkey
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Immunogen
Synthetic peptide corresponding to Mouse S1P1/EDG1 aa 359-372.
Sequence:SHPQKDDGDNPETI
(Peptide available asab39763)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-S1P1/EDG1 antibody (ab11424)Lane 1 : Anti-S1P1/EDG1 antibody (ab11424) at 2 µg/ml
Lane 2 : Anti-S1P1/EDG1 antibody (ab11424) at 2 mg/ml
Lane 1 : Rat brain tissue extract
Lane 2 : Neuro-2a Cells
Secondary
All lanes : Goat anti-rabbit IgG (H+L), HRP conjugated at 0.4 µg/ml
Developed using the ECL technique.
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Immunocytochemistry/ Immunofluorescence - Anti-S1P1/EDG1 antibody (ab11424)Immunocytochemical analysis of Neuro-2a cells, labeling S1P1/EDG1 with ab11424. Cells were fixed in 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. Immunostaining with anti-EDG1 diluted 2µg/ml in 0.1% BSA for 3 hours at room temperature.
Green staining - ab11424
Blue staining - DAPI for nuclear localization
Red staining - F-actin
The right-most image is a control (no primary antibody staining).
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Immunocytochemistry/ Immunofluorescence - Anti-S1P1/EDG1 antibody (ab11424)ab11424 staining S1P1/EDG1 in transfected CCL39 cells by Immunocytochemistry/ Immunofluorescence.
