NADH Assay Kit (Fluorometric, High Sensitivity) (ab234049)
Key features and details
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Cell Lysate, Tissue Lysate
Overview
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Product name
NADH Assay Kit (Fluorometric, High Sensitivity) -
Detection method
Fluorescent -
Sample type
Cell Lysate, Tissue Lysate -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
NADH Assay Kit (Fluorometric, High Sensitivity) (ab234049) provides a highly sensitive method to detect low level NADH in a variety of samples including animal tissues, adherent or suspension cells and enzymatic reactions. The NADH Enzyme Mix specifically recognizes NADH (not NADPH) at levels less than 8nM in the enzyme reaction.
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Notes
NAD and NADH exist in all living organisms and are important for energy transformation and maintenance of the redox state of cells or tissues. The reduced form, NADH is a driving force for cells and plays a critical role in cell regulation and repair processes. It is of increasing interest to be able to measure low level of NADH in samples or in enzymatic reactions.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests NADH Cycling Buffer 1 x 15ml NADH Cycling Enzyme Mix (Lyophilized) 1 vial NADH Extraction Buffer 1 x 50ml NADH Standard (Lyophilized) 1 vial PicoProbe™ (DMSO) 1 x 0.4ml
Images
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NADH Standard curve.
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Decomposition of NADDecomposition of NAD, but not NADH at 60oC for 30 minutes.
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Measurement of NADH in different rat tissues.Measurement of NADH levels (pmol) in rat kidney (1.74 μg), muscle, (2.51 μg), heart (1.59 μg) and liver tissue lysates (29.5 μg).