Anti-TBC1D15 antibody (ab121396)
Key features and details
- Rabbit polyclonal to TBC1D15
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-TBC1D15 antibody
See all TBC1D15 primary antibodies -
Description
Rabbit polyclonal to TBC1D15 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
antigen sequence, corresponding to internal sequence amino acids 169-286 of Human TBC1D15, isoform 2.
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Positive control
- IHC-P: Human placenta, skin, kidney, and testis tissue sections. WB: NIH/3T3, NBT-II, and NTERA-2 cell lysates. ICC/IF: U-2 OS cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBC1D15 antibody (ab121396)
Immunohistochemical analysis of paraffin embedded human kidney tissue labeling TBC1D15 with ab121396 antibody at a 1/20 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunocytochemistry - Anti-TBC1D15 antibody (ab121396)Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoplasm and mitochondria. Recommended concentration of ab121396 1-4 µg/ml. Cells treated with PFA/Triton X-100. -
Western blot - Anti-TBC1D15 antibody (ab121396)All lanes : Anti-TBC1D15 antibody (ab121396) at 0.4 µg/ml
Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate
Lane 2 : NBT-II (rat urinary bladder tumor cell line) cell lysate
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBC1D15 antibody (ab121396)Immunohistochemical analysis of paraffin embedded human testis tissue labeling TBC1D15 with ab121396 antibody at a 1/20 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBC1D15 antibody (ab121396)Immunohistochemical analysis of paraffin embedded human placenta tissue labeling TBC1D15 with ab121396 antibody at a 1/20 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBC1D15 antibody (ab121396)Immunohistochemical analysis of paraffin embedded human skin tissue labeling TBC1D15 with ab121396 antibody at a 1/20 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Western blot - Anti-TBC1D15 antibody (ab121396)Anti-TBC1D15 antibody (ab121396) at 0.4 µg/ml + NTERA-2 (human malignant pluripotent embryonic carcinoma cell line) cell lysate
