Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NME3 antibody [EPR13117] (ab181257)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13117] to NME3
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-NME3 antibody [EPR13117]
    See all NME3 primary antibodies

  • Description

    Rabbit monoclonal [EPR13117] to NME3

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • MOLT4, Raji and HeLa cell lysates; Human cardiac muscle and kidney tissues; HeLa and MOLT4 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NME3 antibody [EPR13117] (ab181257)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NME3 antibody [EPR13117] (ab181257)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling NME3 with ab181257 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-NME3 antibody [EPR13117] (ab181257)
    Immunoprecipitation - Anti-NME3 antibody [EPR13117] (ab181257)

    Western blot analysis of immunoprecipitation pellet from MOLT4 cell lysate immunoprecipitated using ab181257 at 1/50 dilution.
    Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NME3 antibody [EPR13117] (ab181257)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NME3 antibody [EPR13117] (ab181257)

    Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling NME3 with ab181257 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-NME3 antibody [EPR13117] (ab181257)
    Western blot - Anti-NME3 antibody [EPR13117] (ab181257)
    All lanes : Anti-NME3 antibody [EPR13117] (ab181257) at 1/2000 dilution

    Lane 1 : MOLT4 cell lysate
    Lane 2 : Raji cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 19 kDa

  • Anti-NME3 antibody [EPR13117] (ab181257)
    Anti-NME3 antibody [EPR13117] (ab181257)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-NME3 antibody [EPR13117] (ab181257)

© 2021 Astana Biomed Group LLP. All rights reserved.