Anti-TBC1D15 antibody - N-terminal (ab194953)
Key features and details
- Rabbit polyclonal to TBC1D15 - N-terminal
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-TBC1D15 antibody - N-terminal
See all TBC1D15 primary antibodies -
Description
Rabbit polyclonal to TBC1D15 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rabbit, Cat, Chimpanzee, Rhesus monkey, Gorilla, Common marmoset, Orangutan
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Immunogen
Synthetic peptide within Human TBC1D15 aa 1-100 (N terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. (NP_073608.4).
Database link: Q8TC07 -
Positive control
- HeLa, 293T, Jurkat, TCMK-1 and NIH3T3 whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab194953 was affinity purified using an epitope specific to TBC1D15 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TBC1D15 antibody - N-terminal (ab194953)All lanes : Anti-TBC1D15 antibody - N-terminal (ab194953) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK-1 whole cell lysate
Lane 5 : NIH/3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 79 kDa
Exposure time: 30 secondsWhole cell lysates prepared using NETN lysis buffer.
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Immunoprecipitation - Anti-TBC1D15 antibody - N-terminal (ab194953)Detection of TBC1D15 in immunoprecipitates of 293T whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) using ab194953 at 6 µg/mg lysate for IP and at 0.4 µg/ml for subsequent Western blot detection.
Detection: Chemiluminescence with an exposure time of 30 seconds.
