Anti-ACAP2 antibody [EPR15721] (ab191424)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15721] to ACAP2
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-ACAP2 antibody [EPR15721]
See all ACAP2 primary antibodies -
Description
Rabbit monoclonal [EPR15721] to ACAP2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB MouseHuman -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, Jurkat, 293, MOLT4, Human fetal lung, Human fetal kidney, RAW 264.7 and PC12 lysates; Jurkat cells.
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General notes
This product was previously labelled as Centaurin beta 2
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15721 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-ACAP2 antibody [EPR15721] (ab191424) at 1/5000 dilution
Lane 1 : HeLa lysate
Lane 2 : Jurkat lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 88 kDa
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Flow Cytometric analysis of Jurkat (Human T cell leukemia cell line from peripheral blood) cells (paraformaldehyde-fixed, 2%) labeling ACAP2 with ab191424 at 1/270 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
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All lanes : Anti-ACAP2 antibody [EPR15721] (ab191424) at 1/5000 dilution
Lane 1 : 293 lysate
Lane 2 : MOLT4 lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 88 kDa
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All lanes : Anti-ACAP2 antibody [EPR15721] (ab191424) at 1/5000 dilution
Lane 1 : Human fetal lung lysate
Lane 2 : Human fetal kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 88 kDa
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All lanes : Anti-ACAP2 antibody [EPR15721] (ab191424) at 1/1000 dilution
Lane 1 : RAW 264.7 lysate
Lane 2 : PC12 lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 88 kDa
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