Anti-ACAP2 antibody - C-terminal (ab200583)
Images
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Western blot - Anti-ACAP2 antibody - C-terminal (ab200583)All lanes : Anti-ACAP2 antibody - C-terminal (ab200583) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK-1 whole cell lysate
Lane 5 : NIH3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 88 kDa
Exposure time: 3 minutes
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Immunoprecipitation - Anti-ACAP2 antibody - C-terminal (ab200583)Detection of ACAP2 in immunoprecipitates of 293T whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) using ab200583 at 6 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection (Lane 1). Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 30 seconds.
