Antibodies, Proteins, Kits and Reagents for Life Science

307 566 ₸ Product size

100 µg 307 566 ₸ 10 µg 103 862 ₸

Order now and get it on Tuesday March 09, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Mouse monoclonal [98D] to Cdk6
Suitable for: WB
Knockout validated
Reacts with: Mouse, Rat, Human

Product name

Anti-Cdk6 antibody [98D]
See all Cdk6 primary antibodies
Description

Mouse monoclonal [98D] to Cdk6
Host species

Mouse
Tested Applications & Species

Application Species
WB
Mouse

Rat

Human

See all applications and species data
Immunogen

Recombinant fragment within Mouse Cdk6 aa 1-350. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q00534

Run BLAST with Run BLAST with
Positive control
- WB: HeLa, Jurkat, K562, RAW 264.7 and PC-12 whole cell lysates.
General notes
This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Application	Species
WB	Mouse Rat Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

98D
Isotype

IgG1
Research areas

Western blot - Anti-Cdk6 antibody [98D] (ab241554)

All lanes : Anti-Cdk6 antibody [98D] (ab241554) at 1/2000 dilution

Lane 1 : Wild-type HeLa cell lysate
Lane 2 : CDK6 knockout HeLa cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 37 kDa
Observed band size: 38 kDa
why is the actual band size different from the predicted?

Lanes 1 - 2: Merged signal (red and green). Green - ab241554 observed at 38 kDa. Red - loading control ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) observed at 55 kDa.

ab241554 was shown to react with Cdk6 in HeLa wild-type cells in Western blot with loss of signal observed in CDK6 knockout cell line ab266059 (CDK6 knockout cell lysate ab257088). Wild-type HeLa and CDK6 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween^®) before incubation with ab241554 and ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) overnight at 4°C at a 1 in 2000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye^® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye^® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
Western blot - Anti-Cdk6 antibody [98D] (ab241554)

All lanes : Anti-Cdk6 antibody [98D] (ab241554) at 0.518 µg/ml

Lane 1 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
Lane 2 : K-562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate
Lane 3 : PANC-1 (human pancreatic epithelioid carcinoma epithelial cell), whole cell lysate
Lane 4 : Saos-2 (human osteosarcoma epithelial), whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 37 kDa
Observed band size: 37 kDa

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 3 minutes.

Negative control: PANC-1, Saos-2. (PMID: 8114739).
Western blot - Anti-Cdk6 antibody [98D] (ab241554)

All lanes : Anti-Cdk6 antibody [98D] (ab241554) at 0.518 µg/ml

Lane 1 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
Lane 2 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 37 kDa
Observed band size: 37 kDa

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 3 minutes.
Anti-Cdk6 antibody [98D] (ab241554)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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