Anti-Cdk6 antibody (ab54576)
Key features and details
- Mouse monoclonal to Cdk6
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Cdk6 antibody
See all Cdk6 primary antibodies -
Description
Mouse monoclonal to Cdk6 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant fragment: KDGLCRADQQ YECVAEIGEG AYGKVFKARD LKNGGRFVAL KRVRVQTGEE GMPLSTIREV AVLRHLETFE HPNVVRLFDV CTVSRTDRET KLTLVFE, corresponding to amino acids 3-100 of Human Cdk6
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Positive control
- WB: HAP1, HeLa and Jurkat cell lysates.
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General notes
This product was changed from ascites to tissue culture supernatant on 5th December 2019. Lot numbers higher than GR3279055 are from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.4 -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-Cdk6 antibody (ab54576)All lanes : Anti-Cdk6 antibody (ab54576)
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : CDK6 knockout HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 37 kDa
Observed band size: 37 kDaLanes 1- 2: Merged signal (red and green). Green - ab54576 observed at 37 kDa. Red - Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (ab52866) observed at 50 kDa.
ab54576 was shown to react with Cdk6 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab266059 (knockout cell lysate ab257088) was used. Wild-type HeLa and CDK6 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab54576 and Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (ab52866) overnight at 4°C at a 1 µg/ml and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye®800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye®680RD) preadsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Western blot - Anti-Cdk6 antibody (ab54576)Lanes 1-2 : Anti-Cdk6 antibody (ab54576) at 1 µg
Lanes 3-4 : Anti-beta Actin antibody (ab8227) at 1/2000 dilution
Lanes 1 & 3 : Wild-type HAP1 cell lysate
Lanes 2 & 4 : CDK6 knockout HAP1 cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 37 kDaLanes 1 and 2: Green signal from target – ab54576 observed at 37 kDa
Lanes 3 and 4: Red signal from loading control – ab8227 observed at 42 kDa
Lanes 5 and 6: Merged (red and green) signalab54576 was shown to react with CDK6 when CDK6 knockout samples were used, along with additional cross-reactive bands. Wild-type and CDK6 knockout samples were subjected to SDS-PAGE. ab54576 and ab8227 (loading control to beta actin) were diluted 1 µg/mL and 1/2000 respectively and incubated overnight at 4ºC. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
This image was generated using the ascites version of the product.
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Western blot - Anti-Cdk6 antibody (ab54576)Cdk6 antibody (ab54576) at 1ug/lane + Jurkat cell lysate at 25ug/lane.
This image was generated using the ascites version of the product.
