Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Cdk6 antibody [98D] - BSA and Azide free (ab252796)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [98D] to Cdk6 - BSA and Azide free
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Cdk6 antibody [98D] - BSA and Azide free
    See all Cdk6 primary antibodies

  • Description

    Mouse monoclonal [98D] to Cdk6 - BSA and Azide free

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment within Mouse Cdk6 aa 1 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: Q00534

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: HeLa, Jurkat, K562, RAW 264.7 and PC-12 whole cell lysates.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab252796 is the carrier-free version of ab241554. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-Cdk6 antibody [98D] - BSA and Azide free (ab252796)
    Western blot - Anti-Cdk6 antibody [98D] - BSA and Azide free (ab252796)
    All lanes : Anti-Cdk6 antibody [98D] (ab241554) at 1/2000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : CDK6 knockout HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 37 kDa
    Observed band size: 38 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab241554).

    Lanes 1 - 2: Merged signal (red and green). Green - ab241554 observed at 38 kDa. Red - loading control ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) observed at 55 kDa.

    ab241554 was shown to react with Cdk6 in HeLa wild-type cells in Western blot with loss of signal observed in CDK6 knockout cell line ab266059 (CDK6 knockout cell lysate ab257088). Wild-type HeLa and CDK6 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab241554 and ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) overnight at 4°C at a 1 in 2000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

  • Western blot - Anti-Cdk6 antibody [98D] - BSA and Azide free (ab252796)
    Western blot - Anti-Cdk6 antibody [98D] - BSA and Azide free (ab252796)
    All lanes : Anti-Cdk6 antibody [98D] (ab241554) at 0.518 µg/ml

    Lane 1 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
    Lane 2 : K-562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate
    Lane 3 : PANC-1 (human pancreatic epithelioid carcinoma epithelial cell), whole cell lysate
    Lane 4 : Saos-2 (human osteosarcoma epithelial), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 37 kDa
    Observed band size: 37 kDa



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    Negative control: PANC-1, Saos-2. (PMID: 8114739).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab241554).

  • Western blot - Anti-Cdk6 antibody [98D] - BSA and Azide free (ab252796)
    Western blot - Anti-Cdk6 antibody [98D] - BSA and Azide free (ab252796)
    All lanes : Anti-Cdk6 antibody [98D] (ab241554) at 0.518 µg/ml

    Lane 1 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
    Lane 2 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 37 kDa
    Observed band size: 37 kDa



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time:  3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab241554).

  • Anti-Cdk6 antibody [98D] - BSA and Azide free (ab252796)
    Anti-Cdk6 antibody [98D] - BSA and Azide free (ab252796)

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