Recombinant Human VEGFC protein (ab83573)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Suitable for: SDS-PAGE
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Product name
Recombinant Human VEGFC protein
See all VEGFC proteins and peptides -
Purity
> 95 % SDS-PAGE. -
Expression system
HEK 293 cells -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
TheoreticalSequence: FESGLDLSDAEPDAGEATAYASKDLEEQ LRSVSSVDELMTVLYPEYW KMYKCQLRKGGWQHNREQANLNSRTEETI KFAAAHYNTEILKSIDNE WRKTQCMPREVCIDVGKEFGVATNTFFKPP CVSVYRCGGCCNSEGLQ CMNTSTSYLSKTLFEITVPLSQGPKPVTISF ANHTSCRCMSKLDVYR QVHSIIRR Disulfide bonded to: SLPATLPQCQAANKTCPTNYMWNNHICRCLAQEDFMFSSDAGDDS TD GFHDICGPNKELDEETCQCVCRAGLRPASCGPHKELDRNSCQCVCK N KLFPSQCGANREFDENTCQCVCKRTCPRNQPLNPGKCACECTESPQK CLLKGKKFHHQTCSCYRRPCTNRQKACEPGFSYSEEVCRCVPSYWKR PQMS Note: N-terminal sequences confirmed by Edman sequencing.
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Preparation and Storage
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Stability and Storage
Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
Constituents: 10% Trehalose, 1% Human serum albumin
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ReconstitutionIt is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
Images
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Functional Studies - Recombinant Human VEGFC protein (ab83573)The densitometry scan demonstrates the purified human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification. The triangle indicates theoretical pI and MW of the protein.
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Western blot - Recombinant Human VEGFC protein (ab83573) -
SDS-PAGE - Recombinant Human VEGFC protein (ab83573)Lane 1 – MW markers; Lane 2 – ab83573 ; Lane 3 – ab83573 treated with PNGase F to remove potential N-linked glycans; Lane 4 – ab83573 treated with a glycosidase cocktail to remove potential N- and O-linked glycans. Approximately 5 μg of protein was loaded per lane.
Drop in MW after treatment with PNGase F indicates presence of N-linked glycans. A possible further drop in MW after treatment with the glycosidase cocktail indicates the possible presence of O-linked glycans. Additional bands in lane 3 and lane 4 are glycosidase enzymes. -
SDS-PAGE - Recombinant Human VEGFC protein (ab83573)2D SDS-PAGE: A sample of VEGFC without carrier protein was reduced and alkylated and focused on a 3-10 IPG strip then run on a 4-20% Tris-HCl 2D gel. Approximately 40 μg of protein was loaded. Spot train indicates presence of multiple isoforms of VEGFC. Spots within the spot train were cut from the gel and identified as VEGFC by protein mass fingerprinting.
