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Human ANGPTL3 ELISA Kit (ab254510)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 103.25 pg/ml
  • Range: 218.75 pg/ml - 14000 pg/ml
  • Sample type: Cell culture media, EDTA Plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human ANGPTL3 ELISA Kit
    See all ANGPTL3 kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 8 6%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 7.4%

  • Sample type

    Serum, Cell culture media, Hep Plasma, EDTA Plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    103.25 pg/ml

  • Range

    218.75 pg/ml - 14000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 104 100% - 107%
    Cell culture media 101 89% - 114%
    Hep Plasma 95 86% - 103%
    EDTA Plasma 96 83% - 117%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human
    Does not react with: Cow

  • Product overview

    Human ANGPTL3 ELISA Kit (ab254510) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of ANGPTL3 protein in cell culture media, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human ANGPTL3 with 103.25 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    ANGPTL3 (Angiopoietin-like 3) is a glycoprotein made in the liver and involved in lipid metabolism. ANGPTL3 can inhibit endothelial lipase and ANGPTL3 levels correlate with plasma HDL cholesterol, implicating it in modulation of HDL levels. ANGPTL3 expression is also associated with triglyceride levels, as loss of function mutations in ANGPTL3 is often found in individuals with low plasma triglyceride levels. Additionally, interference of ANGPTL3 interactions with LPL (the enzyme that hydrolyzes circulating triglycerides) in mice resulted in a reduction of serum triglyceride levels.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Images

  • SimpleStep ELISA Protocol Diagram
    SimpleStep ELISA Protocol Diagram

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Example of human ANGPTL3 standard curve in Sample Diluent NS
    Example of human ANGPTL3 standard curve in Sample Diluent NS

    The ANGPTL3 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of native ANGPTL3 in human serum and plasma samples.
    Interpolated concentrations of native ANGPTL3 in human serum and plasma samples.

    The concentrations of ANGPTL3 were measured in duplicates, interpolated from the ANGPTL3 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 6.25%, plasma (heparin) 25%, and plasma (EDTA) 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ANGPTL3 concentration was determined to be 96.46 ng/mL in serum, 47.71 ng/mL in plasma (heparin), and 60.52 ng/mL in plasma (EDTA).

  • Serum from ten individual healthy human male donors was measured in duplicate.
    Serum from ten individual healthy human male donors was measured in duplicate.

    Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ANGPTL3 concentration was determined to be 83.11 ng/mL with a range of 39.13 – 196.03 ng/mL.

  • Sandwich ELISA - Human ANGPTL3 ELISA Kit (ab254510)
    Sandwich ELISA - Human ANGPTL3 ELISA Kit (ab254510)
    To learn more about the advantages of recombinant antibodies see here.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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