Anti-ADAM17 antibody (ab2051)
Key features and details
- Rabbit polyclonal to ADAM17
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ADAM17 antibody
See all ADAM17 primary antibodies -
Description
Rabbit polyclonal to ADAM17 -
Host species
Rabbit -
Specificity
80 to 130 kDa bands can be detected, which may represent mature protein, precursor, and glycosylated ADAM17.
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Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human ADAM17 aa 807-823.
Sequence:ASFKLQRQNRVDSKETE
(Peptide available asab7881) -
General notes
TNFa Converting Enzyme.Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western Blot of HeLa (1, 4), Jurkat (2, 5) and Raji (3, 6) cell lysates labeling ADAM17 with Anti-ADAM17 antibody (ab2051) at 0.5µg/ml in the absence (1-3) or presence of blocking peptide (4-6).
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All lanes : Anti-ADAM17 antibody (ab2051) at 0.25 µg/ml
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : TACE KO HeLa cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 93 kDa
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Immunofluorescence of ADAM17 in HeLa cells using ab2051 at 10 ug/ml.
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Lane 1 : Anti-ADAM17 antibody (ab2051) at 0.5 µg/ml
Lane 2 : Unrelated antibody of the same subclass as ab2051.
All lanes : Human keratinocytes (HaCaT)- whole cell lysate from 20000 cells
Secondary
All lanes : HRP conjugated goat polyclonal antibody
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 93 kDa
Observed band size: 100,125 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
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ab2051 at 10µg/ml staining ADAM17 in Hela cells by ICC/IF