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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Other

Anti-ADAM17 antibody (ab2051)

Price and availability

341 740 ₸

Availability

Order now and get it on Wednesday February 24, 2021

Anti-ADAM17 antibody (ab2051)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to ADAM17
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-ADAM17 antibody
    See all ADAM17 primary antibodies
  • Description

    Rabbit polyclonal to ADAM17
  • Host species

    Rabbit
  • Specificity

    80 to 130 kDa bands can be detected, which may represent mature protein, precursor, and glycosylated ADAM17.

  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human ADAM17 aa 807-823.
    Sequence:

    ASFKLQRQNRVDSKETE


    (Peptide available as ab7881)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • General notes

    TNFa Converting Enzyme.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.2
    Preservative: 0.02% Sodium azide
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Other
    • Signal Transduction
    • Adapters
    • Transmembrane
    • Stem Cells
    • Signaling Pathways
    • Notch
    • Secreted
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Extracellular matrix
    • ADAM protein family
    • Cancer
    • Signal transduction
    • Other
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteolytic enzymes
    • Metalloprotease
    • ADAMs
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Neurodegenerative disease
    • Metabolism
    • Types of disease
    • Cancer

Images

  • Western blot - Anti-ADAM17 antibody (ab2051)
    Western blot - Anti-ADAM17 antibody (ab2051)

    Western Blot of HeLa (1, 4), Jurkat (2, 5) and Raji (3, 6) cell lysates labeling ADAM17 with Anti-ADAM17 antibody (ab2051) at 0.5µg/ml in the absence (1-3) or presence of blocking peptide (4-6).

  • Western blot - Anti-ADAM17 antibody (ab2051)
    Western blot - Anti-ADAM17 antibody (ab2051)
    All lanes : Anti-ADAM17 antibody (ab2051) at 0.25 µg/ml

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : TACE KO HeLa cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilution

    Predicted band size: 93 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-ADAM17 antibody (ab2051)
    Immunocytochemistry/ Immunofluorescence - Anti-ADAM17 antibody (ab2051)

    Immunofluorescence of ADAM17 in HeLa cells using ab2051 at 10 ug/ml.

  • Western blot - Anti-ADAM17 antibody (ab2051)
    Western blot - Anti-ADAM17 antibody (ab2051) This image is courtesy of an anonymous Abreview
    Lane 1 : Anti-ADAM17 antibody (ab2051) at 0.5 µg/ml
    Lane 2 : Unrelated antibody of the same subclass as ab2051.

    All lanes : Human keratinocytes (HaCaT)- whole cell lysate from 20000 cells

    Secondary
    All lanes : HRP conjugated goat polyclonal antibody

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 93 kDa
    Observed band size: 100,125 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 minute

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-ADAM17 antibody (ab2051)
    Immunocytochemistry/ Immunofluorescence - Anti-ADAM17 antibody (ab2051)

    ab2051 at 10µg/ml staining ADAM17 in Hela cells by ICC/IF

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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