Anti-Rel B antibody (ab154957)
Key features and details
- Rabbit polyclonal to Rel B
- Suitable for: WB, IHC-P, ChIP, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Rel B antibody
See all Rel B primary antibodies -
Description
Rabbit polyclonal to Rel B -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ChIP, IPmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 354-528 of Human Rel B (Q01201).
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Positive control
- ChIP: THP-1 chromatin extract; WB: Jurkat, transfected HEK-293T, NIH 3T3 whole cell lysates; IHC-P: Human breast carcinoma tissue; IP: HeLa whole cell lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Cross-linked ChIP was performed with THP-1 (human monocytic leukemia cell line) chromatin extract treated with LPS (1.0 μg/ml for 3 h) and 5 μg of either normal rabbit IgG or ab154957. The precipitated DNA was detected by PCR with primer set targeting to IL-1β promoter.
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All lanes : Anti-Rel B antibody (ab154957) at 1/10000 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lane 3 : NCI-H1299 (human lung carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 62 kDa
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue staining Rel B at cytoplasm and nucleus with ab154957 at 1/500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
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RelB antibody immunoprecipitates RelB protein in IP experiments.
IP Sample: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
A. Control with 2 μg of preimmune rabbit IgG
B. Immunoprecipitation of RelB protein by 2 μg of ab154957
7.5% SDS-PAGE
The immunoprecipitated RelB protein was detected by ab154957 at 1/1000 dilution, followed by anti-rabbit IgG antibody.
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All lanes : Anti-Rel B antibody (ab154957) at 1/5000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : Transfected HEK-293T whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 62 kDa7.5% SDS-PAGE
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Anti-Rel B antibody (ab154957) at 1/10000 dilution + NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 62 kDa
7.5% SDS PAGE