Antibodies, Proteins, Kits and Reagents for Life Science

304 886 ₸ Product size

100 µl 304 886 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP613Y] to Rel B
Suitable for: WB, IHC-P, IP, Flow Cyt
Reacts with: Human

Product name

Anti-Rel B antibody [EP613Y]
See all Rel B primary antibodies
Description

Rabbit monoclonal [EP613Y] to Rel B
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human Rel B aa 1-100 (N terminal). The exact sequence is proprietary.
Positive control
- WB: Raji and Daudi cell lysates. IHC-P: Human lymphoma tissue. Flow Cyt: Daudi cells. IP: Raji cells
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EP613Y
Isotype

IgG
Research areas
- Cell Biology
- Apoptosis
- Intracellular
- NFkB
- RelB
- Cancer
- Cell Death
- Apoptosis
- Apoptosis Markers
- NFkB
- RelB

Western blot - Anti-Rel B antibody [EP613Y] (ab33917)

All lanes : Anti-Rel B antibody [EP613Y] (ab33917) at 1/500 dilution

Lane 1 : Raji cell lysate
Lane 2 : Daudi cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP labeled.

Predicted band size: 62 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rel B antibody [EP613Y] (ab33917)

Immunohistochemical analysis of paraffin-embedded human lymphoma using ab33917

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cytometry - Anti-Rel B antibody [EP613Y] (ab33917)

Flow cytometry analysis of Daudi (Human Burkitt's lymphoma lymphoblast) cells labeling Rel B (red) with ab33917 at a 1/2000 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).
Immunoprecipitation - Anti-Rel B antibody [EP613Y] (ab33917)

Purified ab33917 at 1/50 dilution (2µg) immunoprecipitating Rel B in Raji whole cell lysate.
Lane 1 (input): Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate 10µg
Lane 2 (+): ab33917 + Raji whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab33917 in Raji whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 62 kDa
Anti-Rel B antibody [EP613Y] (ab33917)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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