Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD58 antibody [EPR24012-147] (ab275392)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24012-147] to CD58
  • Suitable for: Flow Cyt, WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-CD58 antibody [EPR24012-147]
    See all CD58 primary antibodies

  • Description

    Rabbit monoclonal [EPR24012-147] to CD58

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Raji, K-562, Jurkat and TT whole cell lysates; Human kidney and placenta tissue lysates. Flow cyt: Jurkat and PBMC cells. IP: Raji whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59.94% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR24012-147

  • Isotype

    IgG

  • Research areas

Images

  • Flow Cytometry - Anti-CD58 antibody [EPR24012-147] (ab275392)
    Flow Cytometry - Anti-CD58 antibody [EPR24012-147] (ab275392)

    Flow cytometric analysis of Human peripheral blood mononuclear (PBMC) cells labelling CD58 with ab275392 at 1/500 dilution (0.1ug) (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left).

    Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    Gated on viable cells.

  • Western blot - Anti-CD58 antibody [EPR24012-147] (ab275392)
    Western blot - Anti-CD58 antibody [EPR24012-147] (ab275392)
    All lanes : Anti-CD58 antibody [EPR24012-147] (ab275392) at 1/1000 dilution

    Lane 1 : Human kidney tissue lysate
    Lane 2 : Human placenta tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution

    Predicted band size: 28 kDa
    Observed band size: 55-70 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID:26039213, 3309127, 19268704).

    Exposure time: 3 minutes.

     

  • Immunoprecipitation - Anti-CD58 antibody [EPR24012-147] (ab275392)
    Immunoprecipitation - Anti-CD58 antibody [EPR24012-147] (ab275392)

    CD58 was immunoprecipitated from 0.35 mg Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate with ab275392 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab275392 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate 10 ug

    Lane 2: 275392 IP in Raji whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab275392 in Raji whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 15 seconds.

    This blot was developed using a higher sensitivity ECL substrate.

  • Western blot - Anti-CD58 antibody [EPR24012-147] (ab275392)
    Western blot - Anti-CD58 antibody [EPR24012-147] (ab275392)
    All lanes : Anti-CD58 antibody [EPR24012-147] (ab275392) at 1/1000 dilution

    Lane 1 : Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate at 40 µg
    Lane 2 : A-204 (human muscle rhabdomyosarcoma) whole cell lysate at 40 µg
    Lane 3 : K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 40 µg
    Lane 4 : Jurkat (human t cell leukemia t lymphocyte) whole cell lysate at 20 µg
    Lane 5 : TT (human thyroid carcinoma epithelial cell) whole cell lysate at 20 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution

    Predicted band size: 28 kDa
    Observed band size: 55-70 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID:26039213, 3309127, 19268704).

    Low expression: A-204 (PMID: 26039213).

    Exposure times: Lane 1: 37 seconds; Lanes 2-3: 15 seconds; Lanes 4-5: 70 seconds.

     

  • Flow Cytometry - Anti-CD58 antibody [EPR24012-147] (ab275392)
    Flow Cytometry - Anti-CD58 antibody [EPR24012-147] (ab275392)

    Flow cytometric analysis of A-204 (Human muscle rhabdomyosarcoma, Left) / Jurkat (Human T cell leukemia T lymphocyte, Right) cells labelling CD58 with ab275392 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730)  isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    Low expression control: A-204.

    Gated on viable cells.

  • Anti-CD58 antibody [EPR24012-147] (ab275392)
    Anti-CD58 antibody [EPR24012-147] (ab275392)

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