Anti-ADAM17 antibody (ab57484)
Key features and details
- Mouse monoclonal to ADAM17
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human, Recombinant fragment
- Isotype: IgG2b
Overview
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Product name
Anti-ADAM17 antibody
See all ADAM17 primary antibodies -
Description
Mouse monoclonal to ADAM17 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanWB Recombinant fragment
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Immunogen
Recombinant fragment: RADPDPMKNT CKLLVVADHR FYRYMGRGEE STTTNYLIEL IDRVDDIYRN TSWDNAGFKG YGIQIEQIRI LKSPQEVKPG EKHYNMAKSY PNEEKDAWDV , corresponding to amino acids 215-315 of Human ADAM17
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General notes
This product was changed from ascites to tissue culture supernatant on 25/02/19. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.4 -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-ADAM17 antibody (ab57484)
Western blot against tagged recombinant protein immunogen using ab57484 ADAM17 antibody at 1ug/ml. Predicted band size of immunogen is 37 kD
This image was generated using the ascites version of the product.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM17 antibody (ab57484)ADAM17 antibody (ab57484) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human small Intestine.
This image was generated using the ascites version of the product.
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Flow Cytometry - Anti-ADAM17 antibody (ab57484)Overlay histogram showing HeLa cells stained with ab57484 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab57484, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in HeLa cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.
Please note that Abcam do not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.This image was generated using the ascites version of the product.
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Western blot - Anti-ADAM17 antibody (ab57484) Image courtesy of an anonymous Abreview.Anti-ADAM17 antibody (ab57484) at 1/1000 dilution + whole cell lysate prepared from MCF7 cells at 100000 cells
Secondary
HRP conjugated sheep anti-mouse polyclonal at 1/2000 dilution
Developed using the ECL technique.
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteBlocked with 5% milk for 1 hour at 24°C.
This image was generated using the ascites version of the product.
