Antibodies, Proteins, Kits and Reagents for Life Science

351 792 ₸ Product size

100 µg 351 792 ₸ 10 µg 117 264 ₸

Order now and get it on Tuesday March 16, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Mouse monoclonal [1/MCM6] to MCM6
Suitable for: WB, IHC-P, ICC
Reacts with: Human

Product name

Anti-MCM6 antibody [1/MCM6]
See all MCM6 primary antibodies
Description

Mouse monoclonal [1/MCM6] to MCM6
Host species

Mouse
Tested applications

Suitable for: WB, IHC-P, ICCmore details
Species reactivity

Reacts with: Human
Immunogen

The details of the immunogen for this antibody are not available.
Positive control
- WB: MCF7, HeLa, SW480, U-2 OS and A431, Human tonsil lysates. IHC-P: Human colon and tonsil tissues. ICC: A431, U-2 OS cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

1/MCM6
Isotype

IgG1
Research areas

Western blot - Anti-MCM6 antibody [1/MCM6] (ab280208)

All lanes : Anti-MCM6 antibody [1/MCM6] (ab280208) at 1/1000 dilution

Lane 1 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate
Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell ), whole cell lysate
Lane 3 : SW480 (human colorectal adenocarcinoma epithelial cell), whole cell lysate
Lane 4 : U-2 OS (human bone osteosarcoma epithelial cell), whole cell lysate
Lane 5 : A431 (human epidermoid carcinoma epithelial cell), whole cell lysate
Lane 6 : Human tonsil tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 93 kDa

Blocking and Diluting buffer and concentration: 5% NFDM/TBST

Exposure time lane 1-5: 37 seconds
lane 6: 3 minutes
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM6 antibody [1/MCM6] (ab280208)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling MCM6 with ab280208 at 1/200 (4.945 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Nuclear staining in human colon.The section was incubated with ab280208 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM6 antibody [1/MCM6] (ab280208)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling MCM6 with ab280208 at 1/200 (4.945 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Nuclear staining in human tonsil.The section was incubated with ab280208 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
Immunocytochemistry - Anti-MCM6 antibody [1/MCM6] (ab280208)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized A431 cells labelling MCM6 with ab280208 at 1/20 (49.45 ug/ml) dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear and weakly cytoplasmic staining in A431 cell line. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Immunocytochemistry - Anti-MCM6 antibody [1/MCM6] (ab280208)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized U-2 OS cells labelling MCM6 with ab280208 at 1/20 (49.45 ug/ml) dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear and weakly cytoplasmic staining in U-2 OS cell line. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Anti-MCM6 antibody [1/MCM6] (ab280208)

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