Anti-MEK2 (phospho T394) antibody (ab131095)
Key features and details
- Rabbit polyclonal to MEK2 (phospho T394)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MEK2 (phospho T394) antibody
See all MEK2 primary antibodies -
Description
Rabbit polyclonal to MEK2 (phospho T394) -
Host species
Rabbit -
Specificity
ab131095 detects endogenous levels of MEK2 only when phosphorylated at threonine 394. -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide conjugated to KLH, surrounding the phosphorylation site of Threonine 394 (P-G-Tp-P-T) derived from Human MEK2.
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Positive control
- HepG2 and HeLa cell extracts treated with UV; HeLa cells; Human breast carcinoma tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 0.88% Sodium chloride, 50% Glycerol (glycerin, glycerine)
PBS is without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab131095 was affinity-purified by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-MEK2 (phospho T394) antibody (ab131095) at 1/500 dilution
Lane 1 : HepG2 cell extract, untreated
Lane 2 : HepG2 cell extract, treated with UV
Lane 3 : HeLa cell extract, untreated
Lane 4 : HeLa cell extract, treated with UV
Predicted band size: 44 kDa
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Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labelling MEK2 (phospho T394) with ab131095 antibody at 1/50 dilution. The image on the right was is treated with the synthesized peptide.
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Immunofluorescence of methanol-fixed HeLa cells staining MEK2 (phospho T394) with ab131095 antibody at 1/100 dilution.