Alexa Fluor® 647 Anti-MEK2 antibody [Y78] (ab200519)
![Alexa Fluor® 647 Anti-MEK2 antibody [Y78] (ab200519)](https://www.abcam.com/ps/products/200/ab200519/Images/ab200519-242986-ICCAP002153201.jpg "Alexa Fluor® 647 Anti-MEK2 antibody [Y78] (ab200519)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 647 Rabbit monoclonal [Y78] to MEK2
- Suitable for: ICC/IF
- Knockout validated
- Reacts with: Human
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
Overview
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Product name
Alexa Fluor® 647 Anti-MEK2 antibody [Y78]
See all MEK2 primary antibodies -
Description
Alexa Fluor® 647 Rabbit monoclonal [Y78] to MEK2 -
Host species
Rabbit -
Conjugation
Alexa Fluor® 647. Ex: 652nm, Em: 668nm -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: HeLa and wildtype HAP1 cells.
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General notes
Alternative versions available:
Anti-MEK2 antibody [Y78] (ab32517) - Knockout validatedOur RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
Y78 -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-MEK2 antibody [Y78] (ab200519)
ab200519 staining MEK2 in HeLa cells. The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab200519 at 1/200 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 2µg/ml (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-MEK2 antibody [Y78] (ab200519)](https://www.abcam.com/ps/products/200/ab200519/Images/ab200519-265351-ICCAP002153201.jpg)
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-MEK2 antibody [Y78] (ab200519)ab200519 staining MEK2 in wild-type HAP1 cells (top panel) and MEK2 knockout HAP1 cells (bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab200519 at 1/200 dilution (shown in red) and ab195887 at 1/250 dilution (shown in green) overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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![Alexa Fluor® 647 Anti-MEK2 antibody [Y78] (ab200519)](https://www.abcam.com/ps/products/200/ab200519/Images/ab200519-4-benefits-of-recombinant-antibodies.png)
Alexa Fluor® 647 Anti-MEK2 antibody [Y78] (ab200519)
