Anti-AKT1 + AKT2 + AKT3 antibody (ab126811)
Key features and details
- Rabbit polyclonal to AKT1 + AKT2 + AKT3
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-AKT1 + AKT2 + AKT3 antibody
See all AKT1 + AKT2 + AKT3 primary antibodies -
Description
Rabbit polyclonal to AKT1 + AKT2 + AKT3 -
Host species
Rabbit -
Specificity
Recognizes AKT1, AKT2, and AKT3 proteins -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman
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Immunogen
Synthetic peptide corresponding to Human AKT1 + AKT2 + AKT3 aa 313-317.
Sequence:PEYLA
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Positive control
- 3T6 cell lysate and recombinant Human AKT1, AKT2, and AKT3
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-AKT1 + AKT2 + AKT3 antibody (ab126811) This image is courtesy of an anonymous AbreviewAll lanes : Anti-AKT1 + AKT2 + AKT3 antibody (ab126811) at 1/5000 dilution
All lanes : Rat vascular smooth muscle cell lysate
Lysates/proteins at 100000 cells per lane.
Secondary
All lanes : HRP-conjugated donkey anti-rabbit IgG polyclonal at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 56 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Exposure time: 10 seconds
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Western blot - Anti-AKT1 + AKT2 + AKT3 antibody (ab126811)All lanes : Anti-AKT1 + AKT2 + AKT3 antibody (ab126811) at 1/1000 dilution
Lane 1 : Recombinant Human AKT1
Lane 2 : Recombinant Human AKT2
Lane 3 : Recombinant Human AKT3
Lysates/proteins at 0.015 µg per lane.
Predicted band size: 56 kDa
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Western blot - Anti-AKT1 + AKT2 + AKT3 antibody (ab126811)All lanes : Anti-AKT1 + AKT2 + AKT3 antibody (ab126811) at 1/1000 dilution
Lane 1 : 3T6 cell lysate at 3 µg
Lane 2 : 3T6 cell lysate at 10 µg
Lane 3 : 3T6 cell lysate at 30 µg
Predicted band size: 56 kDa
