Anti-p27 KIP 1 antibody [SX53G8] (ab193379)
![Anti-p27 KIP 1 antibody [SX53G8] (ab193379)](https://www.abcam.com/ps/products/193/ab193379/Images/ab193379-453209-Antip27-KIP-1-antibody-ICC-MCF7.jpg "Anti-p27 KIP 1 antibody [SX53G8] (ab193379)")
Key features and details
- Mouse monoclonal [SX53G8] to p27 KIP 1
- Suitable for: WB, IHC-P, ICC
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-p27 KIP 1 antibody [SX53G8]
See all p27 KIP 1 primary antibodies -
Description
Mouse monoclonal [SX53G8] to p27 KIP 1 -
Host species
Mouse -
Specificity
ab193379 is highly specific and shows no cross-reaction with other related mitotic inhibitors. -
Tested Applications & Species
See all applications and species dataApplication Species ICC HumanIHC-P HumanWB Human
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Immunogen
Recombinant full length protein (proprietary-tag) corresponding to Human p27 KIP 1 aa 1 to the C-terminus.
Database link: P46527 -
Positive control
- WB: HAP1, HeLa and MCF7 cell lysate. IHC: Human colon tissue. ICC: MCF-7 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
SX53G8 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Immunocytochemistry - Anti-p27 KIP 1 antibody [SX53G8] (ab193379)
Immunocytochemical analysis of PFA-fixed MCF-7 cells labeling p27 KIP 1 with ab193379 at 1 µg/mL. A Goat anti-mouse IgG CF488 antibody (Green) was used as a secondary antibody. The membrane is stained with Phalloidin-CF640
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![Western blot - Anti-p27 KIP 1 antibody [SX53G8] (ab193379)](https://www.abcam.com/ps/products/193/ab193379/Images/ab193379-270759-anti-p27-kip-1-antibody-sx53g8-western-blot.jpg)
Western blot - Anti-p27 KIP 1 antibody [SX53G8] (ab193379)Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: CDKN1B knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: MCF7 whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab193379 observed at 30 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab193379 was shown to specifically recognize CDKN1B in wild-type HAP1 cells as well as additional cross-reactive bands. No bands were observed when CDKN1B knockout samples were used. Wild-type and CDKN1B knockout samples were subjected to SDS-PAGE. ab193379 and ab181602 (Rabbit anti GAPDH loading control) were incubated overnight at 4°C at 1 µg/mL and 1/10,000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [SX53G8] (ab193379)](https://www.abcam.com/ps/products/193/ab193379/Images/ab193379-230520-anti-p27-kip-1-antibody-sx53g8-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [SX53G8] (ab193379)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon tissue labeling p27 KIP 1 with ab193379 at 1 µg/mL.
