Anti-MDM2 antibody [EPR22256-98] - BSA and Azide free (ab259275)
![Anti-MDM2 antibody [EPR22256-98] - BSA and Azide free (ab259275)](https://www.abcam.com/ps/products/259/ab259275/Images/ab259275-2-anti-mdm2-antibody-epr22256-98-immunoprecipitation-hepg2-human.jpg "Anti-MDM2 antibody [EPR22256-98] - BSA and Azide free (ab259275)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22256-98] to MDM2 - BSA and Azide free
- Suitable for: ICC/IF, IP, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-MDM2 antibody [EPR22256-98] - BSA and Azide free
See all MDM2 primary antibodies -
Description
Rabbit monoclonal [EPR22256-98] to MDM2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IP, WBmore details
Unsuitable for: Flow Cyt or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: RAW 264.7 treated with 10uM Nutlin-3a for 24 hours whole, 2.4G2 treated with 10uM Nutlin-3a for 24 hours whole lysates. ICC/IF: HepG2 cells treated with 10uM Nutlin-3a for 24 hours. IP: HepG2 treated with 10uM Nutlin-3a for 24 hours whole cell lysate.
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General notes
Ab259275 is the carrier-free version of ab259265. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab259275 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22256-98 -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-MDM2 antibody [EPR22256-98] - BSA and Azide free (ab259275)
MDM2 was immunoprecipitated from 0.35 mg HepG2 (human hepatocellular carcinoma epithelial cell) treated with 10uM Nutlin-3a for 24 hours whole cell lysate 10ug with ab259265 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259265 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.
Lane 1: HepG2 (human hepatocellular carcinoma epithelial cell) treated with 10uM Nutlin-3a for 24 hours whole cell lysate 10ug
Lane 2: ab259265 IP in HepG2 treated with 10uM Nutlin-3a for 24 hours whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab259265 in HepG2 treated with 10uM Nutlin-3a for 24 hours whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.
MDM2 can be cleaved into a 60-kDa fragment after Nutlin 3a treatment as Nutlin 3a disrupts p53–MDM2 interaction and induces p53- dependent apoptosis and autophagy.
The molecular weight observed is consistent with what has been described in the literature (PMID:19638413, 10329737).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab259265).
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![Immunocytochemistry/ Immunofluorescence - Anti-MDM2 antibody [EPR22256-98] - BSA and Azide free (ab259275)](https://www.abcam.com/ps/products/259/ab259275/Images/ab259275-1-anti-mdm2-antibody-epr22256-98-immunocytochemistry-hepg2-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-MDM2 antibody [EPR22256-98] - BSA and Azide free (ab259275)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling MDM2 with ab259265 at 1/100 (5 ug/ml) dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing nuclear staining in HepG2 cells treated with Nutlin-3a (10 uM) for 24 hours is observed. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab259265 anti- MDM2 ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 ug/ml) dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab259265).
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![Anti-MDM2 antibody [EPR22256-98] - BSA and Azide free (ab259275)](https://www.abcam.com/ps/products/259/ab259275/Images/ab259275-3-benefits-of-recombinant-antibodies.png)
Anti-MDM2 antibody [EPR22256-98] - BSA and Azide free (ab259275)
