Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MDM2 antibody [EPR22256-98] - BSA and Azide free (ab259275)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22256-98] to MDM2 - BSA and Azide free
  • Suitable for: ICC/IF, IP, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-MDM2 antibody [EPR22256-98] - BSA and Azide free
    See all MDM2 primary antibodies

  • Description

    Rabbit monoclonal [EPR22256-98] to MDM2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IP, WBmore details
    Unsuitable for: Flow Cyt or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: RAW 264.7 treated with 10uM Nutlin-3a for 24 hours whole, 2.4G2 treated with 10uM Nutlin-3a for 24 hours whole lysates. ICC/IF: HepG2 cells treated with 10uM Nutlin-3a for 24 hours. IP: HepG2 treated with 10uM Nutlin-3a for 24 hours whole cell lysate.

  • General notes

    Ab259275 is the carrier-free version of ab259265. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab259275 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-MDM2 antibody [EPR22256-98] - BSA and Azide free (ab259275)
    Immunoprecipitation - Anti-MDM2 antibody [EPR22256-98] - BSA and Azide free (ab259275)

    MDM2 was immunoprecipitated from 0.35 mg HepG2 (human hepatocellular carcinoma epithelial cell) treated with 10uM Nutlin-3a for 24 hours whole cell lysate 10ug with ab259265 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259265 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: HepG2 (human hepatocellular carcinoma epithelial cell) treated with 10uM Nutlin-3a for 24 hours whole cell lysate 10ug

    Lane 2: ab259265 IP in HepG2 treated with 10uM Nutlin-3a for 24 hours whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab259265 in HepG2 treated with 10uM Nutlin-3a for 24 hours whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds.

    MDM2 can be cleaved into a 60-kDa fragment after Nutlin 3a treatment as Nutlin 3a disrupts p53–MDM2 interaction and induces p53- dependent apoptosis and autophagy.

    The molecular weight observed is consistent with what has been described in the literature (PMID:19638413, 10329737).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab259265).

  • Immunocytochemistry/ Immunofluorescence - Anti-MDM2 antibody [EPR22256-98] - BSA and Azide free (ab259275)
    Immunocytochemistry/ Immunofluorescence - Anti-MDM2 antibody [EPR22256-98] - BSA and Azide free (ab259275)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling MDM2 with ab259265 at 1/100 (5 ug/ml) dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing nuclear staining in HepG2 cells treated with Nutlin-3a (10 uM) for 24 hours is observed. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab259265 anti- MDM2 ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 ug/ml) dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab259265).

  • Anti-MDM2 antibody [EPR22256-98] - BSA and Azide free (ab259275)
    Anti-MDM2 antibody [EPR22256-98] - BSA and Azide free (ab259275)

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