Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MDM2 (phospho S166) antibody [EPR1450(2)] - BSA and Azide free (ab249560)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1450(2)] to MDM2 (phospho S166) - BSA and Azide free
  • Suitable for: Dot blot, IHC-P, WB
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-MDM2 (phospho S166) antibody [EPR1450(2)] - BSA and Azide free
    See all MDM2 primary antibodies

  • Description

    Rabbit monoclonal [EPR1450(2)] to MDM2 (phospho S166) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Dot blot, IHC-P, WBmore details
    Unsuitable for: Flow Cyt,ICC or IP

  • Species reactivity

    Reacts with: Rat, Human
    Does not react with: Mouse

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab249560 is the carrier-free version of ab170880. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab249560 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-MDM2 (phospho S166) antibody [EPR1450(2)] - BSA and Azide free (ab249560)
    Western blot - Anti-MDM2 (phospho S166) antibody [EPR1450(2)] - BSA and Azide free (ab249560)
    All lanes : Anti-MDM2 (phospho S166) antibody [EPR1450(2)] (ab170880) at 1/50000 dilution

    Lane 1 : MCF7 cell lysate
    Lane 2 : MCF7 cell lysate treated with IGF-1

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 55 kDa



    This data was developed using ab170880, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDM2 (phospho S166) antibody [EPR1450(2)] - BSA and Azide free (ab249560)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDM2 (phospho S166) antibody [EPR1450(2)] - BSA and Azide free (ab249560)
    This data was developed using ab170880, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue labeling MDM2 (phospho S166) using ab170880 at a 1/50 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Western blot - Anti-MDM2 (phospho S166) antibody [EPR1450(2)] - BSA and Azide free (ab249560)
    Western blot - Anti-MDM2 (phospho S166) antibody [EPR1450(2)] - BSA and Azide free (ab249560)
    All lanes : Anti-MDM2 (phospho S166) antibody [EPR1450(2)] (ab170880) at 1/100000 dilution

    Lane 1 : A549 (Human lung carcinoma epithelial cell) whole cell lysate.
    Lane 2 : A549 (Human lung carcinoma epithelial cell) treated with insulin at 1ug/ml for 150 minutes. Whole cell lysate.
    Lane 3 : A549 (Human lung carcinoma epithelial cell) treated with insulin at 1ug/ml for 150 minutes. Whole cell lysate. Then the membrane was incubated with phosphatase

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 55 kDa
    Observed band size: 90-140 kDa
    why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    This data was developed using ab170880, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 2% BSA/TBST.

    The molecular weight is the same with the one from this paper PMID: 25392082.

  • Dot Blot - Anti-MDM2 (phospho S166) antibody [EPR1450(2)] - BSA and Azide free (ab249560)
    Dot Blot - Anti-MDM2 (phospho S166) antibody [EPR1450(2)] - BSA and Azide free (ab249560)
    This data was developed using ab170880, the same antibody clone in a different buffer formulation.Dot blot analysis of MDM2 (phospho S166) phospho peptide (Lane 1), MDM2 non-phospho peptide (Lane 2), labeling MDM2 (phospho S166) with ab170880 at a dilution of 1/1000. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/100000. Blocking and dilution buffer: 5% NFDM/TBST. Exposure time: 3 minutes.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDM2 (phospho S166) antibody [EPR1450(2)] - BSA and Azide free (ab249560)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDM2 (phospho S166) antibody [EPR1450(2)] - BSA and Azide free (ab249560)
    This data was developed using ab170880, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling MDM2 (phospho S166) using ab170880 at a 1/50 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Anti-MDM2 (phospho S166) antibody [EPR1450(2)] - BSA and Azide free (ab249560)
    Anti-MDM2 (phospho S166) antibody [EPR1450(2)] - BSA and Azide free (ab249560)

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