Antibodies, Proteins, Kits and Reagents for Life Science

Key features and details

Mouse monoclonal [SMP14] to MDM2
Suitable for: WB, IHC-P
Reacts with: Human
Isotype: IgG1

Product name

Anti-MDM2 antibody [SMP14]
See all MDM2 primary antibodies
Description

Mouse monoclonal [SMP14] to MDM2
Host species

Mouse
Tested applications

Suitable for: WB, IHC-Pmore details
Species reactivity

Reacts with: Human
Immunogen

Synthetic peptide corresponding to Human MDM2 aa 154-167.
Database link: Q00987
Positive control
- WB: HepG2 cell lysate. IHC-P: Human breast carcinoma tissue.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA
Concentration information loading...
Purity

Protein A/G purified
Purification notes

Purified from bioreactor concentrate.
Clonality

Monoclonal
Clone number

SMP14
Isotype

IgG1
Light chain type

kappa
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDM2 antibody [SMP14] (ab263453)

Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for MDM2 using ab263453 at 2 µg/ml in immunohistochemistry.

Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Western blot - Anti-MDM2 antibody [SMP14] (ab263453)

Anti-MDM2 antibody [SMP14] (ab263453) at 2 µg/ml + HepG2 (human liver hepatocellular carcinoma cell line) cell lysate

Predicted band size: 55 kDa

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