Anti-5HT6 Receptor antibody (ab241473)
Key features and details
- Rabbit polyclonal to 5HT6 Receptor
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-5HT6 Receptor antibody
See all 5HT6 Receptor primary antibodies -
Description
Rabbit polyclonal to 5HT6 Receptor -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP Human -
Immunogen
Synthetic peptide within Human 5HT6 Receptor aa 1-50. The exact sequence is proprietary. (NP_000862.1).
Database link: P50406 -
Positive control
- IP: Jurkat whole cell lysate.
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General notes
ab241473 has not performed satisfactorily when used for WB of 5HT6 Receptor in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of 5HT6 Receptor.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab241473 was affinity purified using an epitope specific to 5HT6 Receptor immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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5HT6 Receptor was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241473 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab241473 at 1 µg/ml.
Lane 1: ab241473 IP in Jurkat whole cell lysate.
Lane 2: Control IgG IP in Jurkat whole cell lysate.Detection: Chemiluminescence with exposure time of 30 seconds.
Lysates prepared using RIPA lysis buffer.