Anti-5HT6 Receptor antibody (ab103016)
Key features and details
- Rabbit polyclonal to 5HT6 Receptor
- Suitable for: IHC-P, WB
- Reacts with: Mouse
- Isotype: IgG
Overview
-
Product name
Anti-5HT6 Receptor antibody
See all 5HT6 Receptor primary antibodies -
Description
Rabbit polyclonal to 5HT6 Receptor -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P MouseWB Mouse
-
Immunogen
Synthetic peptide corresponding to Mouse 5HT6 Receptor aa 400 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab117404) -
Positive control
- This antibody gave a positive signal in the following tissue lysates: Mouse dorsal root ganglion; Mouse brain P0; Mouse brain P7. This antibody gave a positive result in IHC in the following FFPE tissue: Mouse Brain.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab103016 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species IHC-P MouseWB MouseApplication Abreviews Notes IHC-P Use a concentration of 0.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 47 kDa).Notes IHC-P
Use a concentration of 0.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.WB
Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 47 kDa).Target
-
Function
This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that stimulate adenylate cyclase. It has a high affinity for tricyclic psychotropic drugs. -
Tissue specificity
Expressed in several human brain regions, most prominently in the caudate nucleus. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
-
Database links
- Entrez Gene: 15565 Mouse
- SwissProt: Q9R1C8 Mouse
- Unigene: 291096 Mouse
-
Alternative names
- 5 HT 6 antibody
- 5 HT6 antibody
- 5 HT6R antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5HT6 Receptor antibody (ab103016)
IHC image of 5HT6 Receptor staining in Mouse Brain formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab103016, 0.5 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
-
Western blot - Anti-5HT6 Receptor antibody (ab103016)All lanes : Anti-5HT6 Receptor antibody (ab103016) at 1 µg/ml
Lane 1 : Dorsal Root Ganglia (Mouse) Tissue Lysate
Lane 2 : P0 Mouse Brain Mouse Tissue Lysate
Lane 3 : P7 Mouse Brain Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 47 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Additional bands at: 41 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
5HT6 Receptor contains a potential glycosylation site (SwissProt) which may explain its migration at a higher molecular weight than predicted.
Protocols
Datasheets and documents
References (5)
ab103016 has been referenced in 5 publications.
- Berthoux C et al. Early 5-HT6 receptor blockade prevents symptom onset in a model of adolescent cannabis abuse. EMBO Mol Med 12:e10605 (2020). PubMed: 32329240
- Teng LL et al. Serotonin receptor HTR6-mediated mTORC1 signaling regulates dietary restriction-induced memory enhancement. PLoS Biol 17:e2007097 (2019). PubMed: 30883547
- Guo J et al. Primary Cilia Signaling Shapes the Development of Interneuronal Connectivity. Dev Cell 42:286-300.e4 (2017). PubMed: 28787594
- Deraredj Nadim W et al. Physical interaction between neurofibromin and serotonin 5-HT6 receptor promotes receptor constitutive activity. Proc Natl Acad Sci U S A 113:12310-12315 (2016). PubMed: 27791021
- Jacobshagen M et al. The serotonin 6 receptor controls neuronal migration during corticogenesis via a ligand-independent Cdk5-dependent mechanism. Development 141:3370-7 (2014). WB, IHC (PFA fixed) ; Mouse . PubMed: 25078650
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5HT6 Receptor antibody (ab103016)
IHC image of 5HT6 Receptor staining in Mouse Brain formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab103016, 0.5 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
-
Western blot - Anti-5HT6 Receptor antibody (ab103016)All lanes : Anti-5HT6 Receptor antibody (ab103016) at 1 µg/ml
Lane 1 : Dorsal Root Ganglia (Mouse) Tissue Lysate
Lane 2 : P0 Mouse Brain Mouse Tissue Lysate
Lane 3 : P7 Mouse Brain Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 47 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Additional bands at: 41 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
5HT6 Receptor contains a potential glycosylation site (SwissProt) which may explain its migration at a higher molecular weight than predicted.
